Journal
/
Bioengineering
/
Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

DOI:
10.3791/59127-v

•

08:11 min

•

January 31, 2019

•

, , , , ,
1Frankfurt Initiative for Regenerative Medicine,Johann Wolfgang Goethe-University, 2J. Crayton Pruitt Family Department of Biomedical Engineering,University of Florida

Chapters

  • 00:04Title
  • 00:46Construction of Electrical Stimulation Cell Culture Chamber
  • 03:22Treating MSCs with EStim
  • 05:48Results: Effect of EStim on MSC Morphology and MSC Osteogenic Marker Gene Expression
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol for the construction of a cell culture chamber designed to expose cells to various types of electrical stimulation, and its use in treating mesenchymal stem cells to enhance osteogenic differentiation.

Tags

Electrical Stimulation ChamberMesenchymal Stem CellsOsteogenic DifferentiationCell BehaviorPlatinum WireSilver-coated WireLEDSix-well Plate LidTerminal Block Connector

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells’ Cardiomyogenic Potential

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Silicon Nanowires and Optical Stimulation for Investigations of Intra- and Intercellular Electrical Coupling

Simple, Affordable, and Modular Patterning of Cells using DNA

Read Article