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Chapters
- 00:04Title
- 01:25Extraction of DNA from Fecal Sample
- 02:2916S rRNA Gene Amplification (PCR1) and DNA Library Construction (PCR2)
- 03:57Clean-up of Indexed PCR (PCR2) and Quantification
- 06:03Sequence Analysis
- 07:49Results: Quality Profile and Statistical Analysis
- 09:29Conclusion
Described here is a simplified standard operating procedure for microbiome profiling using 16S rRNA metagenomic sequencing and analysis using freely available tools. This protocol will help researchers who are new to the microbiome field as well as those requiring updates on methods to achieve bacterial profiling at a higher resolution.
