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JoVE Journal

Immunology and Infection

JoVE Immunology and Infection includes methods and techniques for studying the immune system, mechanisms of infection, biological responses to pathogens, and therapeutic agents for treating immunological disease.

1 2 3 4 5 6 7 8 9…53 › 1,267 VIDEO ARTICLES

Chemical Cartography Approaches to Study Trypanosomatid Infection

Danya A. Dean*^1,2, Jacob J. Haffner*^2,3, Mitchelle Katemauswa¹, Laura-Isobel McCall^1,2,4

¹Department of Chemistry and Biochemistry, University of Oklahoma, Norman, ²Laboratories of Molecular Anthropology and Microbiome Research, University of Oklahoma, Norman, ³Department of Anthropology, University of Oklahoma, Norman, ⁴Department of Microbiology and Plant Biology, University of Oklahoma, Norman

Methods Collection

Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model

Chantelle E Bowers¹, Virginia L Calder¹, John Greenwood¹, Malihe Eskandarpour¹

¹UCL Institute of Ophthalmology, University College London

Methods Collection

C-section of Preclinical Animal Model of Chorioamnionitis Triggered by Group B Streptococcus (GBS)

Taghreed A. Ayash¹, Seline Y. Vancolen², Marie-Julie Allard*¹, Guillaume Sébire*¹

¹Department of Pediatrics, McGill University, ²Department of Pharmacology, McGill University

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

Sarah John¹, Oliver H. Bell², Leslie Wilson¹, David A. Copland², Kathryn L. Pepple¹

¹Department of Ophthalmology, University of Washington, ²Academic Unit of Ophthalmology, Translational Health Sciences, University of Bristol

Methods Collection

Preparation of Pseudo-typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

Xiaoyan Chang*¹, Keru Zhou*¹, Yanguang Liu^1,2, Lian Duan³, Lichun Zhang², Guoliang Zhang³, Haikun Wang⁴, Guiqin Wang¹

¹Nanjing Advanced Academy of Life and Health, ²Institute of Animal Bio-technology, Jilin Academy of Agricultural Sciences, ³National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital, Southern University of Science and Technology, ⁴CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Anthos Christofides^1,2, Carol Cao^1,2,3, Rinku Pal^1,2, Halil I. Aksoylar^1,2, Vassiliki A. Boussiotis^1,2

¹Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, ²Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, ³Harvard College

Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines

Lichen Zhang¹, Rong Huang¹, Liaoxun Lu¹, Rui Fu¹, Guo Guo¹, Yanrong Gu¹, Zhuangzhuang Liu¹, Le He¹, Marie Malissen², Yinming Liang¹

¹The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine, Xinxiang Medical University, ²Centre d’Immunologie de Marseille-Luminy, Aix Marseille Université

Isolation of Primary Mouse Lung Endothelial Cells

Erika Wong^1,2, Nina Nguyen², Judith Hellman²

¹Department of Pediatrics, Division of Critical Care, UCSF Benioff Children's Hospital, ²Department of Anesthesia and Perioperative Care, University of California, San Francisco

Methods Collection

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Shammy Raj¹, Lei Lu², Larry D. Unsworth¹

¹Department of Chemical and Materials Engineering, Donadeo Innovation Centre for Engineering, University of Alberta, ²School of Life Science and Engineering, Southwest Jiaotong University

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice using Reporter-Expressing Recombinant SARS-CoV-2

Desarey Morales Vasquez¹, Kevin Chiem¹, Jesus Silvas¹, Jun-Gyu Park¹, Chengjin Ye¹, Luis Martínez-Sobrido¹

¹Texas Biomedical Research Institute

Plaquing of Herpes Simplex Viruses

Lauren A. Sadowski*¹, Gregory M. Lesko*¹, Chad Suissa*¹, Rista Upadhyay*^1,2, Prashant J. Desai³, Barry J. Margulies^1,4

¹Towson University Herpes Virus Lab, Department of Biological Sciences, Towson University, ²Towson University Department of Chemistry, Towson University, ³Department of Oncology, Johns Hopkins University School of Medicine, ⁴Molecular Biology, Biochemistry, and Bioinformatics Program, Towson University

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

Rafaela S. Fernandes¹, Gabriela D. Noske¹, Victor O. Gawriljuk¹, Ketllyn I. Z. de Oliveira¹, Andre S. Godoy¹, Nathalya C. M. R. Mesquita¹, Glaucius Oliva¹

¹São Carlos Institute of Physics, University of Sao Paulo

Methods Collection

Immunohistochemistry Test for the Lyssavirus Antigen Detection from Formalin-Fixed Tissues

Michael Niezgoda¹, Panayampalli Subbian Satheshkumar¹

¹Poxvirus and Rabies Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Jianmei Xiao^1,2, Xing Wan^1,2, Haoliang Wang^1,2, Guohong Deng^1,2

¹Department of Infectious Diseases, Southwest Hospital, Third Military Medical University (Army Medial University), ²Chongqing Key Laboratory for Research of Infectious Diseases

Drosophila melanogaster Larva Injection Protocol

Ghada Tafesh-Edwards¹, Eric Kenney¹, Ioannis Eleftherianos¹

¹Infection and Innate Immunity Laboratory, Department of Biological Sciences, Institute for Biomedical Sciences, The George Washington University

Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

Kasper Mølgaard*¹, Anne Rahbech*¹, Özcan Met¹, Inge Marie Svane¹, Per thor Straten^1,3, Claus Desler², Marlies J. W. Peeters¹

¹National Center for Cancer Immune Therapy, Department of Oncology, University Hospital Herlev, ²Department of Cellular and Molecular Medicine, Center for Healthy Aging, University of Copenhagen, ³Department of Immunology and Microbiology, Inflammation and Cancer Group, University of Copenhagen

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Delphine M. Depierreux*^1,2, Emily Seshadri*^1,2, Evgeniya V. Shmeleva*^1,2, Jens Kieckbusch^1,2, Delia A. Hawkes¹, Francesco Colucci^1,2

¹Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre, University of Cambridge School of Clinical Medicine, ²Centre for Trophoblast Research, University of Cambridge

Generating Transgenics and Knockouts in Strongyloides Species by Microinjection

Michelle L. Castelletto¹, Elissa A. Hallem^1,2

¹Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, ²Molecular Biology Institute, University of California, Los Angeles

Methods Collection

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Vikramsingh Gujar*¹, Michael B. Anderson*¹, Kenneth E. Miller*¹, Radhika D. Pande¹, Pranav Nawani², Subhas Das¹

¹Department of Anatomy and Cell Biology, Oklahoma State University Center for Health Sciences, ²Institute for Shock Physics, Washington State University

Methods Collection

Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34⁺ Cells Injection, and Cytokine Delivery

Elizabeth Gregorio¹, Eric Ramirez-Salazar¹, Kristen DeRosa¹, Robin Choi¹, Ling Li¹, Meenhard Herlyn¹

¹The Wistar Institute

Isolation of Total RNA from Pseudomonas aeruginosa within Biofilms for Measuring Gene Expression

Kevin Guttman¹, Pauline Wang², Lindsay Jackson¹, Amanda Morris¹, Yvonne Yau³, Valerie Waters^1,4

¹Translational Medicine, Hospital for Sick Children, ²Department of Cell and Systems Biology, University of Toronto, ³Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, ⁴Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals

Tejabhiram Yadavalli¹, Ipsita Volety¹, Deepak Shukla^1,2

¹Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, ²Department of Microbiology and Immunology, University of Illinois at Chicago

Methods Collection

Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung

Ivan V. Maslov¹, Andrey O. Bogorodskiy¹, Mariia V. Pavelchenko¹, Ilia O. Zykov¹, Natalya I. Troyanova², Valentin I. Borshchevskiy^1,3, Marina A. Shevchenko²

¹Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, ²Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ³Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

Jing Yang¹, Qing-Ming Qin¹, Paul de Figueiredo^1,2

¹Department of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center, ²College of Veterinary Medicine, Texas A&M University

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