JoVE Journal

Immunology and Infection

JoVE Immunology and Infection includes methods and techniques for studying the immune system, mechanisms of infection, biological responses to pathogens, and therapeutic agents for treating immunological disease.

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Detection of microRNA Expression in the Kidneys of Immunoglobulin a Nephropathic Mice

Shohei Kaneko¹, Katsunori Yanai¹, Hiroki Ishii¹, Akinori Aomatsu^1,2, Kiyonori Ito¹, Keiji Hirai¹, Susumu Ookawara¹, Kenichi Ishibashi³, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ²Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ³Department of Medical Physiology, Meiji Pharmaceutical University

JoVE 61535

An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract

Ross Monasky¹, Sonia Villa², Shankar Thangamani³

¹College of Veterinary Medicine, Midwestern University, ²Masters in Biomedical Science Program, College of Graduate Studies, Midwestern University, ³Department of Pathology and Population Medicine, College of Veterinary Medicine, Midwestern University

JoVE 61488

Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications

Stephanie Mauti¹, Monique Léchenne², Service Naïssengar³, Abdallah Traoré⁴, Vessaly Kallo^5,6, Casimir Kouakou⁷, Emmanuel Couacy-Hymann⁷, Morgane Gourlaouen⁸, Céline Mbilo^9,10, Pati Patient Pyana¹¹, Enos Madaye³, Ibrahima Dicko⁴, Pascal Cozette¹, Paola De Benedictis⁸, Hervé Bourhy¹, Jakob Zinsstag^9,10, Laurent Dacheux¹

¹Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, ²Environment and Sustainability Institute, University of Exeter, Penryn Campus, ³Institut de Recherche en Elevage pour le Développement, ⁴Laboratoire Central Vétérinaire, ⁵Direction des Services Vétérinaires, ⁶Ecole Inter Etats de Sciences et de Médecine Vétérinaires de Dakar, ⁷Laboratoire Central Vétérinaire de Bingerville, Laboratoire National d'Appui au Développement Agricole Bingerville, ⁸FAO Reference Centre for Rabies, Istituto Zooprofilattico Sperimentale delle Venezie, ⁹Swiss Tropical and Public Health Institute, ¹⁰University of Basel, ¹¹Institut National de Recherche Biomédicale

JoVE 60008

Isolation of Mouse Kidney-Resident CD8⁺ T cells for Flow Cytometry Analysis

Wei Liao^1,2, Chaoyu Ma¹, Nu Zhang¹

¹Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of Texas Health Science Center at San Antonio, ²Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University

JoVE 61559

Methods Collection

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

Meng Wang*¹, Yuanyuan Huang*¹, Wangpeng Gu^2,3, Haikun Wang¹

¹CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences, ²School of Life Science, University of Science and Technology of China, ³State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences

JoVE 60523

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Toni Luise Meister*¹, Mara Klöhn*¹, Eike Steinmann¹, Daniel Todt¹

¹Molecular and Medical Virology, Ruhr University Bochum

JoVE 61373

Optimization of the Cuff Technique for Murine Heart Transplantation

Yunhan Ma*^1,2, Baiyi Xie*^1,2, Helong Dai*^3,4,5, Chenxi Wang⁶, Shujiao Liu⁶, Tianshu Lan⁷, Shuangyue Xu^1,2, Guoliang Yan^1,2,6, Zhongquan Qi^1,2,8

¹Organ Transplantation Institute, School of Medicine, Xiamen University, ²Fujian Key Laboratory of Organ and Tissue Regeneration, ³Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, ⁴Clinical Research Center for Organ Transplantation in Hunan Province, ⁵Clinical Immunology Center, Central South University, ⁶School of Medicine, Xiamen University, ⁷Xiamen Medical College, ⁸Medical College, Guangxi University

JoVE 61103

Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

Kelly L. Monaghan*¹, Breanne Y. Farris*¹, Wen Zheng¹, Edwin C. K. Wan^1,2,3

¹Department of Microbiology, Immunology, and Cell Biology, West Virginia University, ²Department of Neuroscience, West Virginia University, ³Rockefeller Neuroscience Institute, West Virginia University

JoVE 61359

Analysis of Human Natural Killer Cell Metabolism

Javier Traba^1,2, Thomas A. Waldmann³, Olga M. Anton³

¹Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, ²Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), ³Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

JoVE 61466

Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis

Kim Maree O'Sullivan¹, Sarah Creed², Poh-Yi Gan¹, Stephen R. Holdsworth^1,3

¹Department of Medicine, Monash University, ²Monash Micro Imaging, Monash University, ³Immunology Department, Monash Health

JoVE 61180

Production of Germ-Free Fast-Growing Broilers from a Commercial Line for Microbiota Studies

Edouard Guitton¹, Arnaud Faurie¹, Sebastien Lavillatte¹, Thierry Chaumeil¹, Pauline Gaboriaud², Françoise Bussière², Fabrice Laurent², Sonia Lacroix-Lamandé², Rodrigo Guabiraba², Catherine Schouler²

¹INRAE, PFIE, UE-1277, ²INRAE, ISP, Université de Tours

JoVE 61148

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Steven Droho¹, Carla M. Cuda*², Jeremy A. Lavine*¹

¹Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, ²Department of Medicine, Division of Rheumatology, Feinberg School of Medicine, Northwestern University

JoVE 61348

P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

Carlos Victor Montefusco-Pereira*^1,2, Justus C. Horstmann*^1,2, Thomas Ebensen³, Christoph Beisswenger⁴, Robert Bals⁴, Carlos A. Guzmán³, Nicole Schneider-Daum¹, Cristiane de Souza Carvalho-Wodarz¹, Claus-Michael Lehr^1,2

¹Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), ²Department of Pharmacy, Saarland University, ³Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, ⁴Department of Internal Medicine V - Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital

JoVE 61069

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Kathlyn Laval¹, Carola J. Maturana², Lynn W. Enquist¹

¹Department of Molecular Biology, Princeton University, ²Princeton Neuroscience Institute, Princeton University

JoVE 61121

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Raja Veerapandian¹, Anuja Paudyal¹, Adeline Chang¹, Govindsamy Vediyappan¹

¹Division of Biology, Kansas State University

JoVE 61101

Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue

Nikaïa Smith^1,2,3,4, Nassima Bekaddour^2,3,4, Nicolas Leboulanger^4,5, Yolande Richard*⁶, Jean-Philippe Herbeuval*^2,3,4

¹Institute of Molecular Virology, Ulm University Medical Center, ²CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, ³Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, ⁴Université Paris Descartes, Sorbonne Paris Cité, ⁵Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, ⁶Université de Paris, Institut Cochin

JoVE 60914

Isolation of L-form Bacteria from Urine using Filtration Method

Frances Davison¹, Jonathan Chapman¹, Katarzyna Mickiewicz¹

¹Centre for Bacterial Cell Biology, Biosciences Institute, Medical School, Newcastle University

JoVE 61380

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Travis J. Chiarelli¹, Nicole A. Grieshaber¹, Scott S. Grieshaber¹

¹Department of Biological Sciences, University of Idaho

JoVE 61365

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Xiaoyue Sun*^1,2,3, Xiaoqiang Yang*^1,2,3,4, Chui Zeng^1,2,3, Fayed Attia Koutb Megahed^1,2,3,5, Qi Zhou^1,2,3, Tingdang Liu^1,2,3, Waqas Iqbal^1,2,3, Pingnan Sun^1,2,3, Xiaoling Zhou^1,2,3

¹Stem Cell Research Center, Shantou University Medical College, ²The Center for Reproductive Medicine, Shantou University Medical College, ³Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, ⁴Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, ⁵Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications