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JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

1 2 3 4 5 6 7 8 9…90 › 2,137 VIDEO ARTICLES

Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations

Grace A. Snyder¹, William E. Browne², Nikki Traylor-Knowles*¹, Benyamin Rosental*³

¹Department of Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Science, University of Miami, ²Department of Biology, University of Miami, ³The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, and Regenerative Medicine and Stem Cell Research Center, Ben Gurion University of the Negev

Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans

Hanneke Okkenhaug¹, Laetitia Chauve¹, Fatemeh Masoudzadeh¹, Lars Okkenhaug¹, Olivia Casanueva¹

¹Babraham Institute

Methods Collection

Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy

Tomohiro Hirayama*¹, Kyosuke Takabe*², Tatsunori Kiyokawa¹, Nobuhiko Nomura^2,3, Yutaka Yawata*^2,3

¹Graduate School of Life and Environmental Sciences, University of Tsukuba, ²Faculty of Life and Environmental Sciences, University of Tsukuba, ³Microbiology Research Center for Sustainability, University of Tsukuba

Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

Jean-Louis Bru¹, Albert Siryaporn^1,2, Nina Molin Høyland-Kroghsbo³

¹Department of Molecular Biology & Biochemistry, University of California, Irvine, ²Department of Physics & Astronomy, University of California, Irvine, ³Department of Veterinary and Animal Sciences, University of Copenhagen

A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior

Ashley L. Comer^1,2,3,4, Balaji Sriram⁵, William W. Yen², Alberto Cruz-Martín^1,2,3,4,6

¹The Graduate Program for Neuroscience, Boston University, ²Department of Biology, Boston University, ³Neurophotonics Center, Boston University, ⁴Center for Systems Neuroscience, Boston University, ⁵Research and Early Development, Biogen, ⁶Department Pharmacology and Experimental Therapeutics, Boston University

Methods Collection

Measurements of Physiological Stress Responses in C. Elegans

Raz Bar-Ziv*¹, Ashley E. Frakes*¹, Ryo Higuchi-Sanabria*¹, Theodore Bolas¹, Phillip A. Frankino¹, Holly K. Gildea¹, Melissa G. Metcalf¹, Andrew Dillin¹

¹Department of Molecular and Cell Biology, University of California, Berkeley

Methods Collection

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Kelly J. Domesle¹, Shenia R. Young¹, Qianru Yang¹, Beilei Ge¹

¹Center for Veterinary Medicine, U.S. Food and Drug Administration

Tissue-Specific RNAi Tools to Identify Components for Systemic Stress Signaling

Jay Miles¹, Patricija van Oosten-Hawle¹

¹School of Molecular and Cell Biology & Astbury Centre for Structural Molecular Biology, University of Leeds

Methods Collection

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

Diantha van de Vlekkert¹, Xiaohui Qiu¹, Ida Annunziata¹, Alessandra d'Azzo¹

¹Department of Genetics, St. Jude Children's Research Hospital

Simple Detection of Primary Cilia by Immunofluorescence

Alzbeta Filipova¹, Daniel Diaz Garcia², Josef Dvorak³, Stanislav Filip⁴, Marcela Jelicova¹, Zuzana Sinkorova¹

¹Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove, University of Defence, ²Department of Clinical Biochemistry and Diagnostics, University Hospital, ³Department of Oncology, Thomayer Hospital, Charles University, ⁴Department of Oncology and Radiotherapy, Faculty of Medicine, Charles University

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

Hedwig M. Braakhuis¹, Ruiwen He^1,2, Rob J. Vandebriel¹, Eric R. Gremmer¹, Edwin Zwart¹, Jolanda P. Vermeulen¹, Paul Fokkens¹, John Boere¹, Ilse Gosens¹, Flemming R. Cassee^1,2

¹National Institute for Public Health and the Environment (RIVM), ²Institute for Risk Assessment Sciences (IRAS)

Methods Collection

Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series

Jonathan D. Partridge¹, Rasika M. Harshey¹

¹Department of Molecular Biosciences, The University of Texas at Austin

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Jose Meseguer-Ripolles¹, Yu Wang¹, Agnes Sorteberg¹, Aishwariya Sharma², Nan-Linda Ding², Baltasar Lucendo-Villarin¹, Philipp Kramer², Charis-Patricia Segeritz², David C. Hay¹

¹MRC Centre for Regenerative Medicine, University of Edinburgh, ²Research & Development, STEMCELL Technologies Inc

Methods Collection

Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices

Dominik J. Fiegle¹, Tilmann Volk^1,2, Thomas Seidel^1,2

¹Institute of Cellular and Molecular Physiology, Friedrich-Alexander University Erlangen-Nürnberg, ²Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg

Measuring Crop Motility and Food Passaging in Drosophila

Jiadong Cai*^1,2, Junmeng Xi*^1,2, Youheng Wei^1,2

¹Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, ²College of Bioscience and Biotechnology, Yangzhou University

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

Robert Beattie¹, Carmen Streicher¹, Nicole Amberg¹, Giselle Cheung¹, Ximena Contreras¹, Andi H. Hansen¹, Simon Hippenmeyer¹

¹Institute of Science and Technology Austria

Methods Collection

Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy

Łukasz Makowski¹, Damian Trojanowski¹, Jolanta Zakrzewska-Czerwińska¹

¹Department of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Jesús Espada^1,2, Elisa Carrasco³, María I. Calvo-Sánchez^1,4, Sandra Fernández-Martos¹, Juan José Montoya⁵

¹Ramón y Cajal Institute for Health Research (IRYCIS), Ramón y Cajal University Hospital, ²Centro Integrativo de Biología y Química Aplicada (CIBQA), Universidad Bernardo O'Higgins, ³Department of Molecular Biology, Faculty of Sciences, Universidad Autónoma de Madrid (UAM), ⁴Instituto de Investigaciones Biosanitarias, Facultad de Ciencias Experimentales, Universidad Francisco de Vitoria, ⁵Department of Radiology, Rehabilitation & Physiotherapy, Faculty of Medicine, Complutense University

Isolating Myofibrils from Skeletal Muscle Biopsies and Determining Contractile Function with a Nano-Newton Resolution Force Transducer

Martijn van de Locht¹, Josine M. de Winter¹, Dilson E. Rassier², Michiel H.B. Helmes^1,3, Coen A.C. Ottenheijm¹

¹Department of Physiology, Amsterdam UMC, ²Department of Kinesiology and Physical Education, Faculty of Education, McGill University, ³IONOptix BV

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Emily K. Matozel¹, Nathaniel Dale¹, Allen C. Price²

¹Department of Biology, Emmanuel College, ²Department of Chemistry and Physics, Emmanuel College

Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment

Hana Barosova*¹, Barbara Drasler*¹, Alke Petri-Fink¹, Barbara Rothen-Rutishauser¹

¹Adolphe Merkle Institute, University of Fribourg

Methods Collection

In situ Hybridization for Sipunculus nudus Coelomic Fluid

Wenhua Li¹, Mingrui Yuan¹, Yaqin Wu¹

¹Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences, Huaqiao University

Measuring RAN Peptide Toxicity in C. elegans

Paige Rudich¹, Carley Snoznik², Noah Puleo², Todd Lamitina^1,2

¹Graduate Program in Cell Biology and Molecular Physiology, University of Pittsburgh, ²Department of Pediatrics, University of Pittsburgh School of Medicine

Methods Collection

A Finite Element Approach for Locating the Center of Resistance of Maxillary Teeth

Bill Luu¹, Edward Anthony Cronauer², Vaibhav Gandhi¹, Jonathan Kaplan³, David M. Pierce^3,4, Madhur Upadhyay¹

¹Division of Orthodontics, University of Connecticut Health, ²Private Practice, Miami, FL, ³Department of Biomedical Engineering, University of Connecticut, ⁴Department of Mechanical Engineering, University of Connecticut

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