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JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

1 2 3 4 5 6 7 8 9…102 › 2,448 VIDEO ARTICLES

Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins

Krystina Wagner¹, Thomas K. Smylla¹, Matthias Zeger¹, Armin Huber¹

¹Institute of Biology, Department of Biochemistry, University of Hohenheim

Methods Collection

Recording Gap Junction Current from Xenopus Oocytes

Yuan Shui¹, Zhao-Wen Wang¹

¹Department of Neuroscience, University of Connecticut School of Medicine

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Frédérique Lisacek^1,2,3

¹Proteome Informatics Group, SIB Swiss Institute of Bioinformatics, ²Computer Science Department, University of Geneva, ³Section of Biology, University of Geneva

Methods Collection

Longitudinal Intravital Microscopy using a Mammary Imaging Window with Replaceable Lid

Larissa Mourao^1,2, Marta Ciwinska^1,2, Jacco van Rheenen³, Colinda L. G. J. Scheele^1,2

¹Center for Cancer Biology, VIB (BE), ²Department of Oncology, KU Leuven (BE), ³Netherlands Cancer Institute, Department of Molecular Pathology, Oncode Institute (NL)

Methods Collection

Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit Flies

Brook M. Jensen¹, Rachel A. Althoff¹, Sarah E. Rydberg¹, Emma N. Royster¹, Alden Estep², Silvie Huijben¹

¹Center for Evolution and Medicine, School of Life Sciences, Arizona State University, ²United States Department of Agriculture, Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology

Methods Collection

Process Development for the Production and Purification of Adeno-Associated Virus (AAV)2 Vector using Baculovirus-Insect Cell Culture System

Md Nasimuzzaman^1,2, Sophia Villaveces¹, Johannes C. M. van der Loo^1,2,3, Sivani Alla¹

¹Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, ²University of Cincinnati College of Medicine, ³Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia

Single-Particle Interferometric Reflectance Imaging Characterization of Individual Extracellular Vesicles and Population Dynamics

Fengyan Deng¹, Anamika Ratri¹, Clayton Deighan², George Daaboul², Paige C. Geiger¹, Lane K. Christenson¹

¹Department of Molecular and Integrative Physiology, University of Kansas Medical Center, ²NanoView Biosciences

Methods Collection

Organ Culture System for Assessing the Toxicity of Intraocular Treatment Excipients and Pharmaceuticals

Jordan Rossy¹, David J. McCanna², Bernard Fresco³, Jacob G. Sivak¹

¹School of Optometry and Vision Science, University of Waterloo, ²Centre for Ocular Research & Education, School of Optometry and Vision Science, University of Waterloo, ³Tonomed Inc

Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples

Thais A. Correa¹, Fernanda S. Santos², Mariana G. Camargo³, Simone Quinelato², Vânia R. E. P. Bittencourt^1,3, Patricia S. Golo^1,3

¹Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, ²Laboratório de Taxonomia Bioquímica e Bioprospecção de Fungos/Coleção de Cultura de Fungos Filamentosos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), ³Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro

Methods Collection

Optical Clearing of Plant Tissues for Fluorescence Imaging

Daisuke Kurihara¹, Yoko Mizuta^1,2, Shiori Nagahara¹, Yoshikatsu Sato^1,3, Tetsuya Higashiyama^1,3,4

¹Institute of Transformative Bio-Molecules (ITbM), Nagoya University, ²Institute for Advanced Research (IAR), Nagoya University, ³Division of Biological Science, Graduate School of Science, Nagoya University, ⁴Department of Biological Sciences, Graduate School of Science, The University of Tokyo

Methods Collection

Non-thermal Infrared Light Treatment of Ischemia/Reperfusion Injury and Subsequent Analysis of Macrophage Differentiation

Tyler Compton¹, Nicholas Poellinger², Janine Struve³, John G. Krolikowski¹, James T. Ninomiya⁴, Dorothee Weihrauch¹

¹Department of Anesthesiology, Medical College of Wisconsin, ²Department of Internal Medicine, Loyola University Medical Center, ³Department of Orthopedic Surgery, Medical College of Wisconsin, ⁴Department of Orthopedic Surgery, University of New Mexico

Methods Collection

Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies

Sang-Hyuck Park¹, Samuel Koch², Katherine Richardson³, Christopher Pauli¹, Joon-Hee Han⁴, Tae-Hyung Kwon⁴

¹Institute of Cannabis Research, Colorado State University-Pueblo, ²Department of Biology, Colorado State University-Pueblo, ³Department of Chemistry, Colorado State University-Pueblo, ⁴Chuncheon Bioindustry Foundation

Methods Collection

Transverse Fracture of the Mouse Femur with Stabilizing Pin

Emily R. Moore¹, Marina Feigenson², David E. Maridas¹

¹Department of Developmental Biology, Harvard School of Dental Medicine, ²Keros Therapeutics

Methods Collection

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Meng-Fei Xu^1,2, Yu-Hao Yang^1,2, Ya-Lan Wei^3,4, Jing-Lian Zhang^1,2, Xuan Lin^1,2, Xiao-Yan Lin^1,2, Hui Chen^1,2, Zhen-Yu She^1,2

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, ³Fujian Obstetrics and Gynecology Hospital, ⁴Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University

Methods Collection

A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications

Alannah S. Chalkley¹, Margaritha M. Mysior¹, Jeremy C. Simpson¹

¹Cell Screening Laboratory, UCD School of Biology and Environmental Science, University College Dublin

Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

Neelima Boora*¹, Vibha Verma*¹, Ridhi Khurana¹, Gautam Gawande¹, Sanchi Bhimrajka¹, Komal Chaprana¹, Meenu Kapoor², Sanjay Kapoor¹

¹Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, ²University School of Biotechnology, Guru Gobind Singh Indraprastha University

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Yoshika Janapala*¹, Katrina Woodward*¹, Jiwon Lee², Melanie Rug², Thomas Preiss^1,3, Nikolay E. Shirokikh¹

¹Division of Genome Sciences and Cancer, The John Curtin School of Medical Research, The Australian National University, ²Centre for Advanced Microscopy, The Australian National University, ³Victor Chang Cardiac Research Institute

Methods Collection

Protocol for Analysis and Consolidation of TrackMate Outputs for Measuring Two-Dimensional Cell Motility using Nuclear Tracking

Graycen E. Wheeler¹, Amrita Purkayastha¹, Eric N. Bunker¹, David M. Bortz², Xuedong Liu¹

¹Department of Biochemistry, University of Colorado Boulder, ²Department of Applied Mathematics, University of Colorado Boulder

Versatility of Protocols for Resistance Training and Assessment using Static and Dynamic Ladders in Animal Models

Eduardo Iglesias-Gutiérrez^1,2, Manuel Fernández-Sanjurjo², Álvaro F. Fernández³, Francisco José Rodríguez Díaz⁴, Isabel López-Taboada^5,6, Cristina Tomás-Zapico^1,2, Benjamín Fernández-García^2,7

¹Department of Functional Biology, University of Oviedo, ²Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), ³Department of Biochemistry and Molecular Biology, University of Oviedo, ⁴Animal facilities, University of Oviedo, ⁵Department of Psychology, University of Oviedo, ⁶Instituto de Neurociencias del Principado de Asturias (INEUROPA), University of Oviedo, ⁷Department of Morphology and Cellular Biology, University of Oviedo

Methods Collection

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells

Qianqian Guo¹, Kunimaro Furuta¹, Ahmed Aly¹, Samar H. Ibrahim^1,2

¹Division of Gastroenterology & Hepatology, Mayo Clinic, ²Division of Pediatric Gastroenterology, Mayo Clinic

Methods Collection

Spinal Cord Transection In Xenopus laevis Tadpoles

Paula G. Slater¹, Juan Larraín¹

¹Center for Aging and Regeneration, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile

Methods Collection

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Huifei Sophia Zheng¹, Chen-Che Jeff Huang¹

¹Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

Sebastian Bass-Stringer^1,2, Colleen J. Thomas^2,3, Clive N. May³, Paul Gregorevic⁴, Kate L. Weeks^1,5,6, Julie R. McMullen^1,2,5,6,7

¹Baker Heart and Diabetes Institute, ²Department of Physiology, Anatomy and Microbiology, La Trobe University, ³Florey Institute of Neuroscience and Mental Health, University of Melbourne, ⁴Department of Physiology, Centre for Muscle Research (CMR), The University of Melbourne, ⁵Department of Diabetes, Central Clinical School, Monash University, ⁶Baker Department of Cardiometabolic Health, The University of Melbourne, ⁷Department of Physiology and Department of Medicine Alfred Hospital, Monash University

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

Robert C. Moseley¹, Sophia Campione¹, Bree Cummins², Francis Motta³, Steven B. Haase¹

¹Department of Biology, Duke University, ²Department of Mathematical Sciences, Montana State University, ³Department of Mathematical Sciences, Florida Atlantic University

Methods Collection

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