JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

Negin P. Martin*¹, Page Myers*², Eugenia Goulding¹, Shih-Heng Chen¹, Mitzie Walker¹, Thomas M. Porter¹, Lucas Van Gorder¹, Amanda Mathew¹, Artiom Gruzdev³, Erica Scappini⁴, Charles Romeo¹

¹Neurobiology Laboratory, National Institute of Environmental Health Sciences, ²Comparative Medicine Branch, National Institute of Environmental Health Sciences, ³Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, ⁴Signal Transduction Laboratory, National Institute of Environmental Health Sciences

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Jarmila Kralova^1,2, Daniela Glatzova^1,2,3, Simon Borna^1,2, Tomas Brdicka¹

¹Laboratory of Leukocyte Signalling, Institute of Molecular Genetics of the ASCR, ²Faculty of Science, Charles University, ³Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry ASCR

A Plate Competition Assay As a Quick Preliminary Assessment of Disease Suppression

Deborah A. Neher¹, Thomas R. Weicht¹

¹Department of Plant and Soil Science, University of Vermont

Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting

Franziska Jönsson¹, Claudia Hagedorn¹, Florian Kreppel¹

¹Center of Biomedical Education and Research, University Witten/Herdecke

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Ji-Yeon Hyeon¹, David A. Mann¹, Anna M. Townsend¹, Xiangyu Deng¹

¹Center for Food Safety

High-throughput Measurement of Gut Transit Time Using Larval Zebrafish

Steven Cassar¹, Xin Huang², Todd Cole³

¹Preclinical Safety, AbbVie, ²Statistics, AbbVie, ³Integrated Science and Technology, AbbVie

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Ban-Ruo Li*^1,2, Jia Wu*^1,2, Hua-Shan Li^1,2, Zhi-Hui Jiang^1,2, Xiu-Min Zhou^1,2, Cai-Hua Xu^1,2, Ning Ding^1,2, Juan-Min Zha^1,2, Wei-Qi He^1,2

¹Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, ²Department of Oncology, The First Affiliated Hospital of Soochow University

Growing Magnetotactic Bacteria of the Genus Magnetospirillum: Strains MSR-1, AMB-1 and MS-1

Lucas Le Nagard¹, Viviana Morillo-López², Cecile Fradin¹, Dennis A. Bazylinski²

¹Department of Physics & Astronomy, McMaster University, ²School of Life Sciences, University of Nevada Las Vegas

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Hanne M. Hoffmann¹

¹Department of Animal Science, Michigan State University

Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy

Katsuyuki Matsushita¹, Kirsti Golgotiu¹, Daniel J. Orton², Richard D. Smith², Karin D. Rodland², Paul D. Piehowski², Michael P. Hutchens^1,3

¹Anesthesiology & Perioperative Medicine, Oregon Health & Science University, ²Environmental and Biological Services Division, Pacific Northwest National Laboratory, ³Operative Care Division, Portland Veterans Affairs Medical Center

Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography

Ying Zhang*¹, Liming Ma*¹, Xiaodi Pan*¹, Jinglu Wang¹, Xinyu Guo¹, Jianjun Du¹

¹Beijing Key Lab of Digital Plant, Beijing Research Center for Information Technology in Agriculture, Beijing Academy of Agriculture and Forestry Sciences

Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes

Karin Dreisig¹, Frank Wojciechowski Blixt², Karin Warfvinge^1,2

¹Department of Clinical Experimental Research, Glostrup Research Institute, ²Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University

A Versatile Model of Hard Tick Infestation on Laboratory Rabbits

Consuelo Almazán¹, Sarah Bonnet¹, Martine Cote¹, Mirko Slovák², Yoonseong Park³, Ladislav Šimo¹

¹Unité Mixte de Recherche (UMR) Biologie moléculaire et immunologie parasitaires (BIPAR), Institut national de la recherche agronomique (INRA), Ecole Nationale Vétérinaire d'Alfort, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Paris-Est, ²Institute of Zoology, Slovak Academy of Sciences, ³Department of Entomology, Kansas State University

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Maria Felicia Soluri¹, Simone Puccio², Giada Caredda², Giorgio Grillo³, Vito Flavio Licciulli³, Arianna Consiglio³, Paolo Edomi⁴, Claudio Santoro¹, Daniele Sblattero⁴, Clelia Peano^5,6

¹Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, ²Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, ³Institute of Biomedical Technologies, National Research Council, Bari, Italy, ⁴Department of Life Sciences, University of Trieste, Italy, ⁵Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, ⁶Humanitas Clinical and Research Center, Rozzano, Milan, Italy

Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

Elisa Oltra^1,2, Alejandro Caicedo³

¹School of Medicine and Dentistry, Universidad Católica de Valencia San Vicente Mártir, ²Unidad Mixta CIPF-UCV, Centro de Investigación Príncipe Felipe, ³Department of Medicine, University of Miami

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism

Ivanna Karina Olivares-Marin¹, Juan Carlos González-Hernández², Carlos Regalado-Gonzalez¹, Luis Alberto Madrigal-Perez³

¹Department of Chemistry, Universidad Autónoma de Querétaro, ²Department of Biochemical Engineering, Instituto Tecnológico de Morelia, ³Department of Biochemical Engineering, Instituto Tecnológico Superior de Ciudad Hidalgo

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

Alexander Goedel*^1,2, Dorota M. Zawada*¹, Fangfang Zhang¹, Zhifen Chen³, Alessandra Moretti^1,2, Daniel Sinnecker^1,2

¹Medical Department I, University Hospital Klinikum rechts der Isar, Technical University of Munich, ²German Centre for Cardiovascular Research (DZHK), Munich Heart Alliance, ³Beth Israel Deaconess Medical Center, Harvard Medical School

Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells

Katilyn Knutson*¹, Peter R. Strege*¹, Joyce Li², Andrew B. Leiter², Gianrico Farrugia¹, Arthur Beyder¹

¹Enteric Neuroscience Program, Division of Gastroenterology & Hepatology,Department of Physiology & Biomedical Engineering, Mayo Clinic, ²Division of Gastroenterology, Department of Medicine, University of Massachusetts Medical School

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Nikolai Klena*¹, Davide Gambarotto*¹, Maeva Le Guennec¹, Susanne Borgers¹, Paul Guichard¹, Virginie Hamel¹

¹Department of Cell Biology, Sciences III, University of Geneva

High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry

Thomas Williams¹, Robert R. Kay¹

¹MRC-Laboratory of Molecular Biology, Cambridge, UK

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

Anja Baufeld¹, Jens Vanselow¹

¹Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN)

A Proximal Culture Method to Study Paracrine Signaling Between Cells

Subramanyam Dasari¹, Taruni Pandhiri¹, James Haley¹, Dean Lenz², Anirban K. Mitra^1,3,4

¹Indiana University School of Medicine, ²Urology, Indiana University Health Southern Indiana Physicians, ³Indiana University Melvin and Bren Simon Cancer Center, ⁴Department of Medical and Molecular Genetics, Indiana University School of Medicine

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Lisa Couper¹, Andrea Swei¹

¹Department of Biology, San Francisco State University

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

Zhen Wang^1,4, Ramon Ocadiz-Ruiz¹, Sinju Sundaresan¹, Lin Ding¹, Michael Hayes¹, Nirakar Sahoo³, Haoxing Xu^1,2, Juanita L. Merchant^1,2,5

¹Department of Internal Medicine-Gastroenterology, University of Michigan, ²Department of Molecular and Integrative Physiology, University of Michigan, ³Department of Molecular, Cellular and Developmental Biology, University of Michigan, ⁴Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, ⁵Division of Gastroenterology, University of Arizona College of Medicine

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