JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Anna M. Steyer^1,2, Torben Ruhwedel¹, Wiebke Möbius^1,2

¹Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine, ²Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Joslyn Mills¹, Erin McConnell¹, Joshua A. Leitão¹, Louis R. Lapierre¹

¹Department of Molecular Biology, Cell Biology and Biochemistry, Brown University

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

Yung-Chia Chen^1,2, Bu-Miin Huang³

¹Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, ²Department of Anatomy, School of Medicine, Kaohsiung Medical University, ³Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

S. Spencer Scott¹, Xuehui Yang¹, Michael Robich¹, Lucy Liaw¹, Joshua M. Boucher¹

¹Maine Medical Center Research Institute

Medium-throughput Screening Assays for Assessment of Effects on Ca²⁺-Signaling and Acrosome Reaction in Human Sperm

Anders Rehfeld^1,2, Dorte Louise Egeberg Palme^1,2, Kristian Almstrup^1,2, Anders Juul^1,2, Niels Erik Skakkebaek^1,2

¹Department of Growth and Reproduction, Copenhagen University Hospital, ²International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), University of Copenhagen

Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis

Jessica Martinez¹, Viviana Bisbal², Nerea Marin², Antonio Cano^1,3,4, Raul Gómez¹

¹Instituto de Investigación Sanitaria, ²Unidad de Animalario del Centro de Investigación Príncipe Felipe (CIPF), ³Departamento de Pediatría, Obstetricia y Ginecología, Universidad de Valencia, ⁴Servicio de Obstetricia y Ginecología, Hospital Clínico Universitario de Valencia

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Shaarika Sarasija¹, Kenneth R. Norman¹

¹Department of Regenerative and Cancer Cell Biology, Albany Medical College

Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels

Natarajan Perumal*¹, Lars Straßburger¹, Carsten Schmelter¹, Adrian Gericke¹, Norbert Pfeiffer¹, Franz H. Grus¹, Caroline Manicam*¹

¹Department of Ophthalmology, University Medical Centre of the Johannes Gutenberg University Mainz

Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes

Carlos A Brito-Sierra*¹, Jasleen Kaur*¹, Catherine A. Hill^1,2

¹Department of Entomology, Purdue University, ²Purdue Institute for Inflammation, Immunology and Infectious Disease, Purdue University

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting

Svetlana Konovalova¹

¹Research Programs Unit, Molecular Neurology, University of Helsinki

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Carolina Balbi*¹, Sara Bolis*¹, Giuseppe Vassalli^1,2,3, Lucio Barile¹

¹Cellular and Molecular Cardiology Laboratory, Cardiocentro Ticino Foundation, Switzerland, ²Molecular Cardiology Institute, Dept. of Cardiology, University of Zürich, ³Faculty of Biomedical Science, Università Svizzera Italiana, Switzerland

A Versatile Method for Mounting Arabidopsis Leaves for Intravital Time-lapse Imaging

Shigeyuki Betsuyaku^1,2,3,4, Nobuhiko Nomura^3,4, Hiroo Fukuda²

¹Japan Science and Technology Agency (JST), PRESTO, ²Department of Biological Sciences, Graduate School of Science, The University of Tokyo, ³Faculty of Life and Environmental Sciences, University of Tsukuba, ⁴Microbiology Research Center for Sustainability, University of Tsukuba

An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay

Joo Hye Yeo¹, Jinu Lee¹

¹College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Mari Mito^1,2, Mitsutaka Kadota³, Shinichi Nakagawa^1,4, Shintaro Iwasaki^2,5

¹RNA Biology Laboratory, RIKEN, ²RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research, ³Laboratory for Phyloinformatics, RIKEN Center for Biosystems Dynamics Research, ⁴RNA Biology Laboratory, Faculty of Pharmaceutical Sciences, Hokkaido University, ⁵Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo

Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium

Md A. Hakim¹, Erik J. Behringer¹

¹Department of Basic Sciences, Loma Linda University

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Jo Anne Stratton*^1,2,3, Sarthak Sinha*², Wisoo Shin², Elodie Labit², Tak-Ho Chu^1,4, Prajay T. Shah², Rajiv Midha^1,4, Jeff Biernaskie^1,2,3

¹Hotchkiss Brain Institute, University of Calgary, ²Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, ³Alberta Children's Hospital Research Institute, University of Calgary, ⁴Department of Clinical Neurosciences, Cumming School of Medicine, University of Calgary

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca²⁺ Signaling In Situ

Bernard T. Drumm¹, Grant W. Hennig², Salah A. Baker¹, Kenton M. Sanders¹

¹Department of Physiology and Cell Biology, University of Nevada Reno School of Medicine, ²Department of Pharmacology, The Robert Larner, M.D. College of Medicine, University of Vermont

A BW Reporter System for Studying Receptor-Ligand Interactions

Shlomo Elias^1,2, Shira Kahlon², Alexandra Duev-Cohen², Ofer Mandelboim²

¹Department of Hematology, Hadassah - Hebrew University Medical Center, ²The Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, Institute for Medical Research Israel Canada (IMRIC), Hadassah - Hebrew University Medical Center

Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue Samples

Alison M. Moore^1,2, Lee R. Boudreau³, Shakeel Virk³, David P. LeBrun^1,2

¹Department of Pathology and Molecular Medicine, Queen's University, ²Division of Cancer Biology and Genetics, Queen's Cancer Research Institute, ³Queen's Laboratory for Molecular Pathology, Department of Pathology and Molecular Medicine, Queen's University

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)

Madison A. Koon¹, Khadijah Almohammed Ali¹, Robert M. Speaker¹, James P. McGrath¹, Eric W. Linton¹, Michelle L. Steinhilb¹

¹Department of Biology, Central Michigan University

Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors

Maria M. Steele¹, Madeline J. Churchill², Alec P. Breazeale¹, Ryan S. Lane¹, Nicholas A. Nelson¹, Amanda W. Lund^1,2,3,4

¹Department of Cell, Developmental, & Cancer Biology, Oregon Health and Science University, ²Department of Molecular Microbiology & Immunology, Oregon Health and Science University, ³Department of Dermatology, Oregon Health and Science University, ⁴Knight Cancer Institute, Oregon Health and Science University

Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Santosh K. Yadav¹, Paras K. Mishra^1,2

¹Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, ²Department of Anesthesiology, University of Nebraska Medical Center

Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis

Jeffrey R. Millman^1,2, Teresa Doggett³, Christina Thebeau³, Sheng Zhang³, Clay F. Semenkovich¹, Rithwick Rajagopal³

¹Division of Metabolism, Endocrinology and Lipid Research, Department of Medicine, Washington University School of Medicine, ²Department of Biomedical Engineering, Washington University in Saint Louis, ³Department of Ophthalmology and Visual Science, Washington University School of Medicine

Reverse Dissection and DiceCT Reveal Otherwise Hidden Data in the Evolution of the Primate Face

Anne M. Burrows^1,2, Kailey M Omstead¹, Ashley R Deutsch^3,5, Justin T. Gladman⁴, Adam Hartstone-Rose³

¹Department of Physical Therapy, Duquesne University, ²Department of Anthropology, University of Pittsburgh, ³College of Sciences, North Carolina State University, ⁴Shared Materials Instrumentation Facility, Duke University, ⁵Department of Anthropology, University of Florida

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