JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

Lining Wang^1,2, Suyang Zheng^1,2, Yang Guo^1,2, Yalan Pan^1,2, Jie Sun^1,2, Weimin Xu^1,2, Jinlan Lu³, Weidong Li³, Yong Ma^1,2,4

¹Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, ²TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory, Nanjing University of Chinese Medicine, ³Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, ⁴Department of Traumatology and Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine

Intrathoracic Injection for the Study of Adult Zebrafish Heart

Thomas Bise¹, Anna Jaźwińska¹

¹Department of Biology, University of Fribourg

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Jordan L. Ferguson, Heather R. Shive

¹Department of Population Health and Pathobiology, NC State University College of Veterinary Medicine

Induction of Right Ventricular Failure by Pulmonary Artery Constriction and Evaluation of Right Ventricular Function in Mice

Qiancheng Wang¹, Kaitong Chen¹, Hairuo Lin¹, Mingyuan He², Xiaoxia Huang¹, Hailin Zhu¹, Yulin Liao¹

¹Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, ²The Third Clinical Medical College, Guangzhou Medical University

Reproductive Techniques for Ovarian Monitoring and Control in Amphibians

Natalie E. Calatayud^1,2, Norin Chai³, Nicole R. Gardner², Michelle J. Curtis², Monica A. Stoops⁴

¹Reproductive Sciences, San Diego Zoo Institute for Conservation Research, ²Recovery Ecology, San Diego Zoo Institute for Conservation Research, ³Menagerie du Jardin des Plantes, Museum National d'Histoire Naturelle, ⁴Cincinnati Zoo & Botanical Garden, Center for Conservation and Research of Endangered Wildlife

Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Qiushi Xu*¹, Caifeng Wang*¹, Li Ling*¹, Qiuling Yue*¹, Mengrou Liu¹, Shuya Zhang¹, Kaiqiang Fu¹, Lan Ye¹, Ke Zheng¹

¹State Key Laboratory of Reproductive Medicine, Nanjing Medical University

Purification of Platelets from Mouse Blood

Marilou G. Narciso¹, Md Nasimuzzaman^1,2

¹Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, ²University of Cincinnati College of Medicine

Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity

Irene Vorontsova^1,2, James E. Hall¹, Thomas F. Schilling²

¹Department of Physiology and Biophysics, University of California, Irvine, ²Department of Developmental and Cell Biology, University of California, Irvine

4D Microscopy of Yeast

Natalie Johnson¹, Benjamin S. Glick¹

¹Department of Molecular Genetics and Cell Biology, University of Chicago

Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit

Jascha Brettschneider*¹, Jason M. Correnti*¹, Chelsea Lin¹, Bianca Williams¹, Amanke Oranu¹, Amy Kuriakose¹, Dru McIver-Jenkins¹, Abigail Haba¹, Isabelle Kaneza¹, Sookyoung Jeon¹, Eleonora Scorletti¹, Rotonya M. Carr¹

¹Division of Gastroenterology, Perelman School of Medicine, University of Pennsylvania

Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion

Giorgia Careccia*^1,2, Federica Colombo*^1,3, Mario Tirone¹, Alessandra Agresti¹, Marco E. Bianchi^1,2, Samuel Zambrano*^1,2, Emilie Vénéreau*¹

¹Chromatin Dynamics Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) San Raffaele Scientific Institute, ²Vita-Salute San Raffaele University, ³Department of Electronics, Information and Bioengineering, Politecnico di Milano

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

David J. Rademacher¹, Maleen Cabe², Joanna C. Bakowska²

¹Core Imaging Facility and Department of Microbiology and Immunology, Loyola University Chicago, ²Department of Molecular Pharmacology and Therapeutics, Loyola University Chicago

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Anna M. Steyer^1,2, Torben Ruhwedel¹, Wiebke Möbius^1,2

¹Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine, ²Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Joslyn Mills¹, Erin McConnell¹, Joshua A. Leitão¹, Louis R. Lapierre¹

¹Department of Molecular Biology, Cell Biology and Biochemistry, Brown University

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

Yung-Chia Chen^1,2, Bu-Miin Huang³

¹Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, ²Department of Anatomy, School of Medicine, Kaohsiung Medical University, ³Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

S. Spencer Scott¹, Xuehui Yang¹, Michael Robich¹, Lucy Liaw¹, Joshua M. Boucher¹

¹Maine Medical Center Research Institute

Medium-throughput Screening Assays for Assessment of Effects on Ca²⁺-Signaling and Acrosome Reaction in Human Sperm

Anders Rehfeld^1,2, Dorte Louise Egeberg Palme^1,2, Kristian Almstrup^1,2, Anders Juul^1,2, Niels Erik Skakkebaek^1,2

¹Department of Growth and Reproduction, Copenhagen University Hospital, ²International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), University of Copenhagen

Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis

Jessica Martinez¹, Viviana Bisbal², Nerea Marin², Antonio Cano^1,3,4, Raul Gómez¹

¹Instituto de Investigación Sanitaria, ²Unidad de Animalario del Centro de Investigación Príncipe Felipe (CIPF), ³Departamento de Pediatría, Obstetricia y Ginecología, Universidad de Valencia, ⁴Servicio de Obstetricia y Ginecología, Hospital Clínico Universitario de Valencia

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Shaarika Sarasija¹, Kenneth R. Norman¹

¹Department of Regenerative and Cancer Cell Biology, Albany Medical College

Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels

Natarajan Perumal*¹, Lars Straßburger¹, Carsten Schmelter¹, Adrian Gericke¹, Norbert Pfeiffer¹, Franz H. Grus¹, Caroline Manicam*¹

¹Department of Ophthalmology, University Medical Centre of the Johannes Gutenberg University Mainz

Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes

Carlos A Brito-Sierra*¹, Jasleen Kaur*¹, Catherine A. Hill^1,2

¹Department of Entomology, Purdue University, ²Purdue Institute for Inflammation, Immunology and Infectious Disease, Purdue University

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting

Svetlana Konovalova¹

¹Research Programs Unit, Molecular Neurology, University of Helsinki

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Carolina Balbi*¹, Sara Bolis*¹, Giuseppe Vassalli^1,2,3, Lucio Barile¹

¹Cellular and Molecular Cardiology Laboratory, Cardiocentro Ticino Foundation, Switzerland, ²Molecular Cardiology Institute, Dept. of Cardiology, University of Zürich, ³Faculty of Biomedical Science, Università Svizzera Italiana, Switzerland

A Versatile Method for Mounting Arabidopsis Leaves for Intravital Time-lapse Imaging

Shigeyuki Betsuyaku^1,2,3,4, Nobuhiko Nomura^3,4, Hiroo Fukuda²

¹Japan Science and Technology Agency (JST), PRESTO, ²Department of Biological Sciences, Graduate School of Science, The University of Tokyo, ³Faculty of Life and Environmental Sciences, University of Tsukuba, ⁴Microbiology Research Center for Sustainability, University of Tsukuba

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