JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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A Simple Dry Sectioning Method for Obtaining Whole-Seed-Sized Resin Section and Its Applications

Jiajing Qiu*^1,2, Yinhui Ren*^1,2, Lingxiao Zhao^1,2, Biao Zhang^1,2, Cunxu Wei^1,2

¹Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou University, ²Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Joint International Research Laboratory of Agriculture & Agri-Product Safety of the Ministry of Education, Yangzhou University

JoVE 61822

Collection and Identification of Pollen from Honey Bee Colonies

Ellen Topitzhofer¹, Hannah Lucas¹, Emily Carlson¹, Priyadarshini Chakrabarti¹, Ramesh Sagili¹

¹Department of Horticulture, Oregon State University

JoVE 62064

Methods Collection

Three-Dimensional Adipocyte Culture as a Model to Study Cachexia-Induced White Adipose Tissue Remodeling

Miguel L Batista Jr.^1,2, Tova Meshulam^2,3, Kathleen Desevin², Nabil Rabhi², Stephen R. Farmer²

¹Department of Integrated Biotechnology, University of Mogi das Cruzes, ²Department of Biochemistry, Boston University School of Medicine, ³Obesity Research Center, Department of Medicine, Boston University School of Medicine

JoVE 61853

Methods Collection

Dry Root Rot Disease Assays in Chickpea: a Detailed Methodology

Vadivelmurugan Irulappan¹, Muthappa Senthil-Kumar¹

¹National Institute of Plant Genome Research

JoVE 61702

Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana

Aigerim S. Kamzina*^1,2,3, Michelle P. DiPalma*^1,2,3, Joseph G. L. Hunter^1,2,3, Andrew G. Diamos^1,2,4, Boyd Armer^2,3, Tsafrir S. Mor^1,2,3, Hugh S. Mason^1,2,3

¹Center for Immunotherapy, Vaccines and Virotherapy, The Biodesign Institute, Arizona State University, ²School of Life Sciences, Arizona State University, ³Molecular Biosciences/Biotechnology Undergraduate Program, Arizona State University, ⁴Department of Microbiology and Immunology, University of Michigan Medical School

JoVE 61774

MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T

Remco van Schadewijk¹, Julia R. Krug^2,3,4, Andrew Webb⁵, Henk Van As^2,4, Aldrik H. Velders^3,4, Huub J. M. de Groot¹, A. Alia^1,6

¹Solid-state NMR, Leiden Institute of Chemistry, Faculty of Science, Leiden University, ²Laboratory of Biophysics, Wageningen University & Research, ³Laboratory of BioNanoTechnology, Wageningen University & Research, ⁴MAGNEtic resonance Facility, Wageningen University & Research, ⁵C.J. Gorter Center for High Field MRI, Radiology department, Leiden University Medical Centre, Leiden University, ⁶Institute for Medical Physics and Biophysics, Leipzig University

JoVE 61266

Bovine Ovarian Cortex Tissue Culture

Courtney M. Sutton*¹, Shelby A. Springman*¹, Mohamed A. Abedal-Majed², Andrea S. Cupp¹

¹Department of Animal Science, University of Nebraska-Lincoln, ²Department of Animal Production, School of Agriculture, University of Jordan

JoVE 61668

Laser Capture Microdissection on Surgical Tissues to Identify Aberrant Gene Expression in Impaired Wound Healing in Type 2 Diabetes

Rachael Williams*¹, Irene Castellano-Pelicena*^1,2, Aaiad H.A Al-Rikabi^1,3, Stephen K. Sikkink¹, Richard Baker¹, Kirsten Riches-Suman¹, M Julie Thornton¹

¹Centre for Skin Sciences, University of Bradford, ²The Charles Institute of Dermatology, School of Medicine, University College Dublin, ³University of Baghdad

JoVE 62091

Methods Collection

Super-Resolution Live Cell Imaging of Subcellular Structures

Rajesh Ranjan¹, Xin Chen¹

¹Department of Biology, The Johns Hopkins University

JoVE 61563

Specimen Collection and Analysis of the Duodenal Microbiome

Benjamin W. Dreskin¹, Kayti Luu¹, Tien S. Dong^1,2,3, Jihane Benhammou^1,2,4, Venu Lagishetty^2,3, John Vu¹, Daniel Sanford¹, Francisco Durazo^2,4, Vatche G. Agopian⁴, Jonathan P. Jacobs^1,2,3, Joseph R. Pisegna^1,5, Shehnaz K. Hussain⁶

¹Division of Gastroenterology, Hepatology and Parenteral Nutrition, Veterans Administration Greater Los Angeles Healthcare System, ²The Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at University of California, ³UCLA Microbiome Center, David Geffen School of Medicine at University of California, ⁴Department of Surgery, University of California, Los Angeles, ⁵Department of Medicine and Human Genetics, David Geffen School of Medicine at University of California, ⁶Cedars-Sinai Cancer and Department of Medicine, Cedars-Sinai Medical Center

JoVE 61900

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Kelly Veerasammy*^1,2, Yuki X. Chen*^1,2, Sami Sauma*¹, Mathilde Pruvost¹, David K. Dansu¹, Tenzin Choetso^1,2, Tiffany Zhong³, Damien Marechal¹, Patrizia Casaccia¹, Rinat Abzalimov^4,5, Ye He^1,5

¹The Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York, ²The City College of New York, CUNY, ³The Bronx High School of Science, ⁴The Graduate Center - Advanced Science Research Center, Structural Biology Initiative, The City University of New York, ⁵The Graduate Center - Advanced Science Research Center, MALDI MS Imaging Joint Core Facility, The City University of New York

JoVE 62008

In vivo Evaluation of Mucociliary Clearance in Mice

Kyle S. Feldman¹, Maliha Zahid¹

¹Department of Developmental Biology, University of Pittsburgh School of Medicine

JoVE 61929

Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)

Muhammad Rahmad Royan¹, Shinji Kanda², Daichi Kayo³, Weiyi Song⁴, Wei Ge⁴, Finn-Arne Weltzien¹, Romain Fontaine¹

¹Physiology Unit, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, ²Laboratory of Physiology, Atmosphere and Ocean Research Institute, The University of Tokyo, ³Department of Biological Sciences, Graduate School of Science, The University of Tokyo, ⁴Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau

JoVE 62006

Tethered Bilayer Lipid Membranes to Monitor Heat Transfer between Gold Nanoparticles and Lipid Membranes

Amani Alghalayini^1,2, Lele Jiang¹, Xi Gu³, Guan Heng Yeoh³, Charles G. Cranfield^1,2, Victoria Timchenko³, Bruce A. Cornell^2,4, Stella M. Valenzuela^1,2,5

¹School of Life Sciences, University of Technology Sydney, ²ARC Research Hub for Integrated Devices for End-user Analysis at Low-levels (IDEAL), Faculty of Science, University of Technology Sydney, ³School of Mechanical and Manufacturing Engineering, The University of New South Wales, ⁴Surgical Diagnostics Pty Ltd., ⁵Institute for Biomedical Materials and Devices, University of Technology Sydney

JoVE 61851

Methods Collection

Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-like Cells using the Three-dimensional Co-culture System, NICO-1

Yuko Miyagawa¹, Kazunori Nagasaka¹, Kaoru Yamawaki^2,3, Yutaro Mori^2,3, Tatsuya Ishiguro², Kei Hashimoto¹, Ryoko Koike¹, Siho Fukui¹, Takeru Sugihara¹, Takayuki Ichinose¹, Haruko Hiraike¹, Koichiro Kido¹, Koji Okamoto³, Takayuki Enomoto², Takuya Ayabe¹

¹Department of Obstetrics and Gynecology, Teikyo University School of Medicine, ²Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, ³Devision of Cancer Differentiation, National Cancer Center Research Institute

JoVE 61751

Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis

Elena Dalla Benetta*¹, Duverney Chaverra-Rodriguez*¹, Jason L. Rasgon², Omar S. Akbari¹

¹Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, ²Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences, The Pennsylvania State University

JoVE 61892

Preparation of a User-Defined Peptide Gel for Controlled 3D Culture Models of Cancer and Disease

Jennifer C. Ashworth¹, Rebecca L. Morgan¹, Kataryna Lis-Slimak¹, Kate A. Meade¹, Sal Jones¹, Katherine Spence², Charlotte E. Slater¹, Jamie L. Thompson¹, Anna M. Grabowska¹, Robert B. Clarke², Gilian Farnie³, Cathy L. R. Merry¹