JoVE Journal

Biology

JoVE Biology publishes standard techniques and novel experimental approaches in the fields of cellular, molecular, and organismal biology aimed at understanding the functions of life and living organisms.

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Precise, High-throughput Analysis of Bacterial Growth

Masaomi Kurokawa¹, Bei-Wen Ying^1,2

¹Graduate School of Life and Environmental Sciences, University of Tsukuba, ²Institute of Biology and Information Science, East China Normal University

Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets

Madina Makhmutova¹, Tao Liang², Herbert Gaisano², Alejandro Caicedo¹, Joana Almaça¹

¹Department of Medicine, University of Miami, ²Department of Medicine, University of Toronto

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Gail M Nelson¹, Jenna M Guynn², Brian N Chorley¹

¹National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, ²Oak Ridge Institute for Science and Education at U.S. Environmental Protection Agency

Study of Protein-protein Interactions in Autophagy Research

Secil Erbil-Bilir¹, Nur M. Kocaturk¹, Melih Yayli², Devrim Gozuacik^1,3

¹Molecular Biology, Genetics and Bioengineering Program, Sabanci University, ²Information Center, Sabanci University, ³EFSUN, Center of Excellence for Functional Surfaces and Interfaces for Nano Diagnostics, Sabanci University

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

Shane C. McAllister¹, Ryan L. Hanson¹, Kyleen N. Grissom¹, Sara Botto², Ashlee V. Moses²

¹Division of Pediatric Infectious Diseases, University of Minnesota Medical School, ²Vaccine and Gene Therapy Institute, Oregon Health and Science University

Volume Segmentation and Analysis of Biological Materials Using SuRVoS (Super-region Volume Segmentation) Workbench

Michele C. Darrow¹, Imanol Luengo^1,2, Mark Basham¹, Matthew C. Spink¹, Sarah Irvine¹, Andrew P. French², Alun W. Ashton¹, Elizabeth M.H. Duke¹

¹Science Division, Harwell Science and Innovation Campus, Diamond Light Source, ²School of Computer Science, University of Nottingham

Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus

Hae-Na Chung¹, Stacy D. Rodriguez¹, Victoria K. Carpenter², Julia Vulcan¹, C. Donovan Bailey¹, Madhugiri Nageswara-Rao¹, Yiyi Li³, Geoffrey M. Attardo⁴, Immo A. Hansen^1,5

¹Department of Biology, New Mexico State University, ²Department of Molecular Genetics and Microbiology, Duke University, ³Department of Computer Sciences, New Mexico State University, ⁴Department of Epidemiology of Microbial Diseases, Yale School of Public Health, ⁵Institute of Applied Biosciences, New Mexico State University

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

Christopher Weidner¹, Matthias Steinfath¹, Elisa Wistorf¹, Michael Oelgeschläger¹, Marlon R. Schneider¹, Gilbert Schönfelder^1,2

¹Department of Experimental Toxicology and ZEBET, German Federal Institute for Risk Assessment (BfR), ²Department of Clinical Pharmacology and Toxicology, Charité-Universitätsmedizin Berlin

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Thomas R. Vincent¹, James Canham¹, Masatsugu Toyota^2,3,4, Marieta Avramova¹, Sam T. Mugford⁵, Simon Gilroy², Anthony J. Miller¹, Saskia Hogenhout⁵, Dale Sanders¹

¹Department of Metabolic Biology, John Innes Centre, Norwich Research Park, ²Department of Botany, University of Wisconsin, Madison, ³Department of Biochemistry and Molecular Biology, Saitama University, ⁴Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), ⁵Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

Perran A. Ross¹, Jason K. Axford¹, Kelly M. Richardson¹, Nancy M. Endersby-Harshman¹, Ary A. Hoffmann¹

¹School of BioSciences, Bio21 Institute and University of Melbourne

Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator

Adam J. Rossano¹, Michael F. Romero^1,2

¹Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, ²Department of Nephrology and Hypertension, Mayo Clinic College of Medicine

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Hieng Chiong Tie¹, Bing Chen¹, Xiuping Sun¹, Li Cheng^2,3, Lei Lu¹

¹School of Biological Sciences, Nanyang Technological University, ²Bioinformatics Institute, ³School of Computing, National University of Singapore

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

James S. Byers¹, Daniel F. Jarosz^1,2

¹Department of Developmental Biology, Stanford University School of Medicine, ²Department of Chemical and Systems Biology, Stanford University School of Medicine

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Chrstine Bäuerl*¹, Özgun C.O. Umu*², Pablo E. Hernandez³, Dzung B. Diep⁴, Gaspar Pérez-Martínez¹

¹Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), ²Department of Food Safety and Infection Biology, Norwegian University of Life Sciences (NMBU), ³Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid (UCM), ⁴Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

Naeem Nathoo^1,2, Mark A. Bernards², Jacqueline MacDonald³, Ze-Chun Yuan^1,3

¹London Research and Development Centre, Agriculture & Agri-Food Canada, ²Department of Biology, University of Western Ontario, ³Department of Microbiology and Immunology, University of Western Ontario

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Haley R. Pugsley¹

¹Amnis Biology Department, MilliporeSigma

Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.

Cui Cao¹, Wen J. Wang¹, Ying Y. Huang¹, Hong L. Yao¹, Louis P. Conway¹, Li Liu¹, Josef Voglmeir¹

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Fengwu Li¹, Christopher A. Adase¹, Ling-juan Zhang¹

¹Department of Dermatology, School of Medicine, UC San Diego

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Tanumoy Saha¹, Isabel Rathmann¹, Milos Galic¹

¹DFG Cluster of Excellence 'Cells in Motion', (EXC 1003), Institute of Medical Physics and Biophysics, University of Muenster

Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

Arjan D. Barendrecht*¹, Johan J. F. Verhoef*², Silvia Pignatelli¹, Gerard Pasterkamp¹, Harry F. G. Heijnen¹, Coen Maas¹

¹Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, ²Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

Thurston Herricks¹, Fred D. Mast^1,2, Song Li¹, John D. Aitchison^1,2

¹Institute for Systems Biology, ²Center for Infectious Disease Research

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Colleen M. Garvey¹, Torin A. Gerhart¹, Shannon M. Mumenthaler¹

¹Lawrence J. Ellison Institute for Transformative Medicine, University of Southern California (USC)

Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects

Yoko Miura^1,2,3, Joachim Pruessner¹, Carla Lotta Mertineit¹, Katharina Kern¹, Michael Muenter¹, Moritz Moltmann², Veit Danicke², Ralf Brinkmann^1,2

¹Institute of Biomedical Optics, University of Luebeck, ²Medical Laser Center Luebeck GmbH, University of Leubeck, ³Department of Ophthalmology, University of Luebeck

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Stephanie Chanon¹, Christine Durand¹, Aurelie Vieille-Marchiset¹, Maud Robert², Charna Dibner³, Chantal Simon¹, Etienne Lefai¹

¹CarMeN Laboratory, INSERM U1060, INRA 1397, University of Lyon, ²Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon, Lyon 1 University, ³Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine, University of Geneva

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