Drosophila melanogaster Ovary Dissection: A Technique for Ex Vivo Tissue Preparation

Published: April 30, 2023

Abstract

Source: Parker, D. J., et al. Studying Mitochondrial Structure and Function in Drosophila Ovaries. J. Vis. Exp. (2017).

This video describes the general anatomy of the Drosophila ovary and features a method of dissecting ovaries for live or fixed imaging.

Protocol

This protocol is an excerpt from Parker et al., Studying Mitochondrial Structure and Function in Drosophila Ovaries, J. Vis. Exp. (2017). 1. Preparation of Drosophila (the tools required are depicted in Figure 1A) For any of the experiments described, collect Drosophila (maintained at room temperature, or 25 °C) within 5 days of eclosion and place them in a vial filled with 5 …

Representative Results

Figure 1: Experimental Plan and Preparation. (A) Tools used in the methods described: A. Fly vial; B. Insect dissecting medium; C. Paraformaldehyde; D. Fly brush; E. Dissecting dish; F. Cover glass; G. Microfuge tube. H. Glass-bottomed dish; I. Glass slide; J. 1,000-µL micropipette; K. 200-µL micropipette; L. 2.5-µL micropipette; M. Teasing needle with a bent tip (tip …

Materials

Grace's Media (Insect Dissecting Medium) Fisher Scientific 30611031-2
41 Paraformaldehyde AQ Electronic Microscopy Sciences 50-259-99
PolyLysine MP Biomedicals ICN15017625
Fly Vials Fisher Scientific AS-515
Fly Vial Plugs Fisher Scientific AS273
Jazzmix Drosophila food (Drosophila food) Fisher Scientific AS153
Azer Scientific EverMark Select Microscope Slides Fisher Scientific 22-026-252
Microscope Cover Glass Fisher Scientific 12-542-B
Mat Tek Corp Glass Bottom Mircrowell Dish Fisher Scientific P35G-0-14-C
Active Dried Yeast Fisher Scientific ICN10140001
Dumont #5 Forceps Fine Science Technologies 11251-20
Moria Nickel Plated Pin Holder Fine Science Technologies 26016-12
Minutien Pins Fine Science Technologies 26002-15
MYFP ( w[*]; P{w[+mC]=sqh-EYFP-Mito}3 ) Bloomington Stock Center 7194
Fly Pad Fly stuff 59-118
Blowgun Fly stuff 54-104
Blowgun needle Flystuff 54-119
Dissecting Microscope Carl Zeiss Stemi 2000

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Cite This Article
Drosophila melanogaster Ovary Dissection: A Technique for Ex Vivo Tissue Preparation. J. Vis. Exp. (Pending Publication), e20101, doi: (2023).

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