Preparation of Drosophila Heart: A Technique to Study Heart Physiology in the Adult Fly

Published: April 30, 2023

Abstract

Source: Vogler, G., Ocorr, K. Visualizing the Beating Heart in Drosophila. J. Vis. Exp. (2009).

This video describes the fly's adult heart, or otherwise called the Drosophila dorsal vessel. The featured protocol demonstrates a dissection procedure for live heart preparations.

Protocol

This protocol is an excerpt from Vogler and Ocorr, Visualizing the Beating Heart in Drosophila, J. Vis. Exp. (2009). 1. Before you start Freshly prepare artificial hemolymph (AH) solution containing 108mM Na+, 5mM K+, 2mM Ca2+, 8mM MgCl2, 1mM NaH2PO4, 4mM NaHCO3, 10mM sucrose, 5mM trehalose, 5mM HEPES (pH 7.1, all reagents from Sigma Chemicals). The sucrose and trehalose sho…

Materials

Micro Dissecting Spring Scissors (curved) Roboz Surgical Instruments Co. RS-5611 Good for gross cuts (Step 2)
Micro Dissecting Spring Scissors (straight) Roboz Surgical Instruments Co. RS-5620
Dumont #55 forceps Fine Science Tools 11295-51
Dumont #5 forceps Fine Science Tools 11295-10
Glass Capillaries, fine Science Products GB100T8P
Pipette Puller Sutter Equipment P-97 Both horizontal and vertical pipette pullers will work
Plastic tubing Tygon 1/16" inner diameter for 100 µl capillaries
Histoacryl® tissue adhesive B. Braun Medical

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Cite This Article
Preparation of Drosophila Heart: A Technique to Study Heart Physiology in the Adult Fly. J. Vis. Exp. (Pending Publication), e20151, doi: (2023).

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