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Biology
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现场共聚焦显微镜对斑马鱼的胚胎脑成像
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Biology
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JoVE Journal
Biology
Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
现场共聚焦显微镜对斑马鱼的胚胎脑成像
DOI:
10.3791/1217-v
•
07:11 min
•
April 01, 2009
•
Ellie Graeden
2
,
Hazel Sive
2
1
Department of Biology
,
MIT – Massachusetts Institute of Technology
,
2
Whitehead Institute for Biomedical Research
,
MIT – Massachusetts Institute of Technology
Chapters
00:00
Title
00:37
Introduction
01:09
Preparing for Injection
02:14
Injecting mRNA
04:12
Mounting and Imaging Embryos
06:14
Representative Results: Confocal Imaging
06:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这段视频中,我们表现出的方法,通过它来分析生活在单细胞共聚焦显微镜分辨率的斑马鱼胚胎发育中的脊椎动物大脑。这包括我们注入单细胞的斑马鱼胚胎的方法,通过它,随后装载和图像的大脑发育。
Tags
Live Imaging
Zebrafish Embryonic Brain
Confocal Microscopy
Cell Shape Changes
Cell Rearrangements
Zebrafish Brain Development
Neural Tube Morphogenesis
Vertebrate Brain Structure
Membrane-targeted Green Fluorescent Protein (memGFP)
Agarose Mounting
Fluorescent Imaging
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