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JoVE Journal Biology
Split-Ubiquitin Basierend Membrane Yeast Two-Hybrid (Mythos) System
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Split-Ubiquitin Basierend Membrane Yeast Two-Hybrid (Mythos) System: Ein wirksames Instrument zur Ermittlung von Protein-Protein-Wechselwirkungen

Article DOI: 10.3791/1698 • 14:04 min • February 1st, 2010
February 1st, 2010 •
1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Biochemistry, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Terrence Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto

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MYTHOS ermöglicht den empfindlichen Nachweis von transienten und stabilen Wechselwirkungen zwischen Proteinen, die im Modellorganismus Saccharomyces cerevisiae exprimiert werden. Es wurde erfolgreich angewendet, um exogene und Hefe integrale Membranproteine ​​zu studieren, um ihre Interaktionspartner in einem hohen Durchsatz Weise zu identifizieren.

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Cellular Biology Ausgabe 36 Protein-Protein Interaktion Membran- Split-Ubiquitin- Hefe- Bibliotheks-Screening Y2H Hefe-Zwei-Hybrid Mythos
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