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基于分体式泛素膜酵母双杂交（神话）系统：一个强大的工具用于识别蛋白质 - 蛋白质相互作用

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Biology

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JoVE Journal Biology

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

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基于分体式泛素膜酵母双杂交（神话）系统：一个强大的工具用于识别蛋白质 - 蛋白质相互作用

30,752 Views

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14:04 min

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February 01, 2010

DOI:

DOI:

•

14:04 min

February 01, 2010

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26 Views

Jamie Snider^1,2,3, Saranya Kittanakom^1,2,3, Jasna Curak^1,2,3, Igor Stagljar^1,2,3

¹Department of Biochemistry,University of Toronto, ²Department of Molecular Genetics,University of Toronto, ³Terrence Donnelly Centre for Cellular and Biomolecular Research (CCBR),University of Toronto

Summary

神话允许的瞬时和稳定的互动模式生物酵母中表达的蛋白质之间的灵敏的检测。它已成功地应用于研究外源性和酵母的完整的膜蛋白，以确定其在高通量的方式相互作用的合作伙伴。

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