JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
基于分体式泛素膜酵母双杂交(神话)系统:一个强大的工具用于识别蛋白质 - 蛋白质相互作用
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
Please note that all translations are automatically generated.
Click here for the English version.
基于分体式泛素膜酵母双杂交(神话)系统:一个强大的工具用于识别蛋白质 - 蛋白质相互作用
DOI:
10.3791/1698-v
•
14:04 min
•
February 01, 2010
•
Jamie Snider
2,3
,
Saranya Kittanakom
2,3
,
Jasna Curak
2,3
,
Igor Stagljar
2,3
1
Department of Biochemistry
,
University of Toronto
,
2
Department of Molecular Genetics
,
University of Toronto
,
3
Terrence Donnelly Centre for Cellular and Biomolecular Research (CCBR)
,
University of Toronto
Chapters
00:00
Title
01:22
Introduction
01:41
Selection of Bait and Appropriate MYTH System
03:04
Generation of tMYTH Baits by Gap Repair
03:07
a. Vector Preparation and Amplification of Gene of Interest
05:30
b. Yeast Transformation and Recovery of Bait Plasmid DNA
07:03
Bait Validation
08:10
MYTH Screening
08:12
a. Large Scale Yeast Transformation
11:31
b. Bait Dependency Test
12:54
Representative Results/ Outcome
13:12
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
神话允许的瞬时和稳定的互动模式生物酵母中表达的蛋白质之间的灵敏的检测。它已成功地应用于研究外源性和酵母的完整的膜蛋白,以确定其在高通量的方式相互作用的合作伙伴。
Tags
Split-ubiquitin
Membrane Yeast Two-hybrid
Protein-protein Interactions
MYTH System
Full-length Transmembrane Proteins
Saccharomyces Cerevisiae
Bait Protein
Prey Proteins
Ubiquitin Moieties
Artificial Transcription Factor
Pseudo-ubiquitin Molecule
Deubiquitinating Enzymes
Reporter Gene Expression
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
成像蛋白质 - 蛋白质相互作用
在体内</em
确定远距离的DNA相互作用的相关染色体陷阱
改良酵母双杂交系统,以确定蛋白与生长因子Progranulin的相互作用
一个协议,用于基于蛋白质 - 蛋白质相互作用的鉴定<sup> 15</sup> N代谢标记,免疫沉淀法,的定量质谱法和亲和调制
鉴定蛋白质 - 蛋白质相互作用的<em>果蝇</em>成人头由串联亲和纯化(TAP)
反向酵母双杂交系统,以确定哺乳动物核受体残留的互动与配体和/或拮抗剂
捕获复合质谱 - 一个强大的工具来确定新型C-二GMP效应蛋白
研究蛋白质 - 蛋白质相互作用活细胞使用生物发光共振能量转移
探讨高密度功能蛋白微阵列来检测蛋白质 - 蛋白质相互作用
鉴定蛋白质相互作用的网站使用多肽阵列
Read Article