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在斑马鱼胚胎的单个神经元和神经嵴细胞的细胞运动和肌动蛋白细胞骨架的实时成像
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JoVE Journal
Biology
Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
Please note that all translations are automatically generated.
Click here for the English version.
在斑马鱼胚胎的单个神经元和神经嵴细胞的细胞运动和肌动蛋白细胞骨架的实时成像
DOI:
10.3791/1726-v
•
10:52 min
•
February 03, 2010
•
Erica Andersen
2,3
,
Namrata Asuri
2,3
,
Matthew Clay
3,4
,
Mary Halloran
2,3,4
1
Genetics Training Program
,
University of Wisconsin-Madison
,
2
Department of Anatomy
,
University of Wisconsin-Madison
,
3
Department of Zoology
,
University of Wisconsin-Madison
,
4
Cell and Molecular Biology Training Program
,
University of Wisconsin-Madison
Chapters
00:00
Title
00:47
Introduction
01:18
Assembling Injection Slides and Imaging Slides
02:36
Injecting DNA Constructs
04:43
Mounting Embryos for Imaging
06:52
4D Imaging of Cell Behaviors on Olympus FV1000 Confocal with IX81 Microscope
08:49
Behavior of Individual Labeled Neurons and Neural Crest Cells
10:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了生活的斑马鱼胚胎的单个神经元或神经嵴细胞成像。使用此方法来研究细胞行为和肌动蛋白定位使用荧光共焦时间推移显微镜。
Tags
Live Imaging
Cell Motility
Actin Cytoskeleton
Individual Neurons
Neural Crest Cells
Zebrafish Embryos
Development
In Vivo
Optical Clarity
High Resolution Imaging
Cell Behaviors
Migration
Axon Outgrowth
Guidance
Mechanisms
Cell Labeling
Plasmid DNA Injection
Transient Mosaic Expression Pattern
Protrusive Activity
Molecular Dynamics
Transgenic Lines
Fine Protrusions
Changes In Molecular Distribution
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