JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
細胞の電気融合を蛍光顕微鏡で可視化
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Cell Electrofusion Visualized with Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
細胞の電気融合を蛍光顕微鏡で可視化
DOI:
10.3791/1991-v
•
05:02 min
•
July 01, 2010
•
Katja Trontelj
,
Marko Ušaj
,
Damijan Miklavčič
1
Faculty of Electrical Engineering
,
University of Ljubljana
Chapters
00:00
Title
00:20
Loading the Cells with Cell Trackers CMFDA and CMRA
02:31
Electrofusion
03:39
Image Acquistion and Determination of the Fusion Yield
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
このビデオでは、細胞の効率的な電気融合を示しています<em> in vitroで</em>エレクトロポレーションおよび蛍光顕微鏡による融合細胞の可視化のその後の検出を使用して変更密着法による。
Tags
Cell Electrofusion
Fluorescence Microscopy
Hybrid Cells
Human Therapy
Non-viral Method
Non-chemical Method
High-voltage Electric Pulses
Cell Plasma Membranes
Permeability
Fusion
Lipid Bilayers
Cytoplasm
Adherence Method
Cell Tracker Dyes
Orange CMRA
Green CMFDA
Fusion Yield
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
蛍光顕微鏡による可視化単分子のDNAの複製
個々のシナプス前終末でのドーパミン放出はFFNsと可視化
siRNAトランスフェクション後の有糸分裂のタイムラプスイメージング
生細胞におけるストア作動性カルシウムエントリの蛍光ベースの測定:培養癌細胞から骨格筋線維に
分裂酵母の定量的なライブセル蛍光-顕微鏡解析
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
タバコプロトプラストと葉で二分子蛍光相補性によって可視化し、タンパク質間相互作用
単一のビリオン原子間力顕微鏡と超解像蛍光イメージングのサンプル調製
蛍光顕微鏡でストロミュール頻度を可視化
ゲルの表面上に成長する植物の根の光シート蛍光顕微鏡
Read Article