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Microarrays을 사용하여 tRNA의 Aminoacylation (충전) 레벨의 게놈 - 와이드 분석
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays
DOI:

07:32 min

June 18, 2010

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Chapters

  • 00:00Title
  • 00:45Introductions
  • 01:06Isolation of Total Charged tRNA
  • 02:37Cy3/Cy5 Labeling of tRNA
  • 05:15Hybridization and Analysis of the Microarray
  • 06:33Representative Results of Isolation and Analysis of tRNA
  • 07:09Conclusion

Summary

Automatic Translation

우리는 모든 tRNAs의 상대 aminoacylation 수준을 결정하기 위해 microarray 분석을위한 방법을 설명<em> S. cerivisiae.</em

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