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Journal
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Immunology and Infection
/
可视化的细胞与细胞转移的艾滋病毒,艾滋病毒和现场共聚焦显微镜的荧光克隆
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
可视化的细胞与细胞转移的艾滋病毒,艾滋病毒和现场共聚焦显微镜的荧光克隆
DOI:
10.3791/2061-v
•
13:08 min
•
October 07, 2010
•
Benjamin Dale
,
Gregory P. McNerney
,
Deanna L. Thompson
,
Wolfgang Hübner
,
Thomas Huser
,
Benjamin K. Chen
1
Division of Infectious Diseases, Department of Medicine, Immunology Institute
,
Mount Sinai School of Medicine
,
2
NSF Center for Biophotonics
,
University of California, Davis
,
3
Structural and Computational Biology Unit
,
European Molecular Biology Laboratory
Chapters
00:00
Title
00:55
Introduction
01:26
Preparing the Donor (Jurkat) and Target (Primary) CD4+ T cells
03:47
Cleaning Up the Donor Cells and Labeling the Target Cells
06:36
Monitor Cell-to-cell Transfer of HIV Gag-iGFP Using Live Cell Transfer
12:02
Representative Images of Cell-to-cell Transfer of HIV
12:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这种可视化的实验是利用荧光艾滋病毒的分子克隆,现场共聚焦成像实验的指导。
Tags
Visualizing
Cell-to-cell Transfer
HIV
Fluorescent Clones
Live Confocal Microscopy
Green Fluorescent Protein
Complex Cellular Processes
Fluorescence Video Microscopy
Human Immunodeficiency Virus Core Protein
Gag Protein
Infectious Molecular Clone
HIV Gag-iGFP
Video Confocal Microscopy
Transfect
Human T Cell Line
Fluorescently Labeled Uninfected CD4+ T Cells
Target Cells
Viral Production
Transport
Intercellular Structures
Virological Synapses
Gas Permeable Imaging Chambers
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