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Journal
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Neuroscience
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在子宫内
的电穿孔其次原发性神经文化为研究基因的功能在皮层神经元的一个子集
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
In utero
Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
Please note that all translations are automatically generated.
Click here for the English version.
在子宫内
的电穿孔其次原发性神经文化为研究基因的功能在皮层神经元的一个子集
DOI:
10.3791/2103-v
•
08:24 min
•
October 08, 2010
•
Heather Rice
,
Seiyam Suth
,
William Cavanaugh
,
Jilin Bai
,
Tracy L. Young-Pearse
1
Center for Neurologic Diseases
,
Brigham and Woman’s Hospital and Harvard Medical School
,
2
Department of Physiology and Neurobiology
,
University of Connecticut
Chapters
00:00
Title
00:21
Supscription Lock
00:56
Introduction
01:49
Injecting DNA and Electroporation
05:36
Analyzing Neuronal Process Outgrowth
06:22
Analysis of the Cell Types of Electroporated Neurons
07:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在子宫内
电转染神经祖细胞
在体内
的一个有价值的方法。根据电极和电发育时间点的位置,才能有的放矢。皮层细胞的某些子集靶细胞,然后
可以在体内或
体外基因改变的影响进行分析。
Tags
In Utero Electroporation
Primary Neuronal Culture
Gene Function
Subset Of Cortical Neurons
Neurite Outgrowth
Genetic Alterations
ShRNA
CDNA Constructs
Chemical Transfection
Viral Transduction
Heterogeneous Pool Of Cell Types
Glutamatergic Neurons
Inhibitory Neurons
Glial Cells
Embryonic Rodent Cortex
Deep Layers Of The Cortex
Superficial Layers Of The Cortex
Dorsal-medial Regions Of The Cortex
Ventral-lateral Regions Of The Cortex
Quantitative Analysis
Step-by-step Method
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