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Biology
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细胞的细胞大分子运输检测
在植物
利用基因枪法
JoVE Journal
Biology
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JoVE Journal
Biology
A Cell-to-cell Macromolecular Transport Assay
in Planta
Utilizing Biolistic Bombardment
Please note that all translations are automatically generated.
Click here for the English version.
细胞的细胞大分子运输检测
在植物
利用基因枪法
DOI:
10.3791/2208-v
•
07:14 min
•
August 27, 2010
•
Shoko Ueki
,
Benjamin L. Meyers
,
Farzana Yasmin
,
Vitaly Citovsky
1
Department of Biochemistry and Cell Biology
,
State University of New York at Stony Brook
,
2
Bio-Medical Engineering Department
,
NED University of Engineering and Technology
Chapters
00:00
Title
00:51
Introduction
01:24
Target Plant Growth
02:32
Biolistic Delivery of DNA-coasted Microparticles
04:09
Imaging of Protein Expression
05:17
Representative Results for a Cell-to-cell Macromelucular Transport Assay
06:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
高分子植物细胞之间的贩运,可以通过瞬时表达利益荧光标记的蛋白质,分析其内部和细胞间共聚焦显微镜的分布进行评估。
Tags
Cell-to-cell Macromolecular Transport
Assay
Biolistic Bombardment
Fluorescently Tagged Protein
Encoding DNA Construct
Confocal Microscopy
Symplastic Protein Transport
Arabidopsis Thaliana
Nicotiana Benthamiana
N. Tabacum (tobacco)
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