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Ein Cell-to-cell Makromolekulare Transport Assay In Planta Verwendung Biolistic Bombardment
JoVE Journal
Biology
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JoVE Journal Biology
A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
DOI:

07:14 min

August 27, 2010

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Chapters

  • 00:00Title
  • 00:51Introduction
  • 01:24Target Plant Growth
  • 02:32Biolistic Delivery of DNA-coasted Microparticles
  • 04:09Imaging of Protein Expression
  • 05:17Representative Results for a Cell-to-cell Macromelucular Transport Assay
  • 06:38Conclusion

Summary

Automatic Translation

Makromolekulare Handel zwischen Pflanzenzellen können durch transiente Expression eines Fluoreszenz-markierten Proteins von Interesse und die Analyse ihrer intra-und interzelluläre Verteilung durch konfokale Mikroskopie beurteilt werden.

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