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Chapters
- 00:05Title
- 01:29Sample Fixation and Resin Embedding
- 04:00Preparing the Sample for the FIB/SEM
- 05:55Imaging the FIB/SEM
- 08:03Results: FIB/SEM Images of Brain Tissue
- 08:40Conclusion
Tags
Focussed Ion Beam MillingScanning Electron MicroscopyFIB/SEM ProtocolAldehydes FixationHeavy Metal StainingOsmium TetroxideUranyl AcetateDehydrationResin InfiltrationUltramicrotomeGlass KnivesBlock PreparationVertical Face MillingBackscattered Electron ImagingSerial ImagingIsotropic VoxelsCell Ultrastructure Analysis
