Journal
/
/
Analisando a função de GTPases Pequenas pela microinjeção de Plasmids em Polarized Células Epiteliais
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
DOI:

09:38 min

May 31, 2011

, , ,

Chapters

  • 00:05Title
  • 01:27Preparation of DNA and MDCK Cells
  • 02:04Microinjection Procedure and Post-injection Incubations
  • 06:07Surface Staining for Immunofluorescence Analysis
  • 08:21VSVGts045-GFP Control in MDCK Cells
  • 09:05Conclusion

Summary

Automatic Translation

Este artigo detalha os procedimentos envolvidos na análise da superexpressão e GTPases pequenas células epiteliais polarizadas utilizando a técnica de microinjeção.

Related Videos

Read Article