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Polarize Epitel Hücreleri içine Plazmidler, Mikroenjeksiyon Küçük GTPases Fonksiyon Analizi
JoVE Journal
Biology
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JoVE Journal Biology
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
DOI:

09:38 min

May 31, 2011

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Chapters

  • 00:05Title
  • 01:27Preparation of DNA and MDCK Cells
  • 02:04Microinjection Procedure and Post-injection Incubations
  • 06:07Surface Staining for Immunofluorescence Analysis
  • 08:21VSVGts045-GFP Control in MDCK Cells
  • 09:05Conclusion

Summary

Automatic Translation

Bu makalede, mikroenjeksiyon tekniği kullanarak polarize epitel hücreleri küçük GTPases aşırı ekspresyonu ve analiz yer alan usul.

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