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为极化上皮细胞注射质粒的小GTP酶的功能分析
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Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
为极化上皮细胞注射质粒的小GTP酶的功能分析
DOI:
10.3791/2645-v
•
09:38 min
•
May 31, 2011
•
Rita Nokes Cook
,
Su Fen Ang*
1
,
Richard Seung-on Kang*
1
,
Heike Fölsch
1
Department of Cell and Molecular Biology
,
Northwestern University
Chapters
00:05
Title
01:27
Preparation of DNA and MDCK Cells
02:04
Microinjection Procedure and Post-injection Incubations
06:07
Surface Staining for Immunofluorescence Analysis
08:21
VSVGts045-GFP Control in MDCK Cells
09:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文详细介绍了在极化上皮细胞,利用显微注射技术的小GTP酶的过度表达和分析所涉及的程序。
Tags
Small GTPases
Microinjection
Plasmids
Polarized Epithelial Cells
Apical Domain
Basolateral Domain
Tight Junctions
MDCK Cells
Cell Polarity
Ras Superfamily
RalA
Cdc42
Rab8
Rab10
Rab13
GDP-bound State
GTP-bound State
Nucleotide Binding Regions
Dominant Negative
Dominant Active Alleles
Overexpression
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