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为极化上皮细胞注射质粒的小GTP酶的功能分析
JoVE Journal
Biology
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JoVE Journal Biology
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
DOI:

09:38 min

May 31, 2011

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Chapters

  • 00:05Title
  • 01:27Preparation of DNA and MDCK Cells
  • 02:04Microinjection Procedure and Post-injection Incubations
  • 06:07Surface Staining for Immunofluorescence Analysis
  • 08:21VSVGts045-GFP Control in MDCK Cells
  • 09:05Conclusion

Summary

Automatic Translation

本文详细介绍了在极化上皮细胞,利用显微注射技术的小GTP酶的过度表达和分析所涉及的程序。

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