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Biology
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편광 상피 세포에 Plasmids의 Microinjection에 의해 작은 GTPases의 기능을 분석
JoVE Journal
Biology
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JoVE Journal
Biology
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
편광 상피 세포에 Plasmids의 Microinjection에 의해 작은 GTPases의 기능을 분석
DOI:
10.3791/2645-v
•
09:38 min
•
May 31, 2011
•
Rita Nokes Cook
,
Su Fen Ang*
1
,
Richard Seung-on Kang*
1
,
Heike Fölsch
1
Department of Cell and Molecular Biology
,
Northwestern University
Chapters
00:05
Title
01:27
Preparation of DNA and MDCK Cells
02:04
Microinjection Procedure and Post-injection Incubations
06:07
Surface Staining for Immunofluorescence Analysis
08:21
VSVGts045-GFP Control in MDCK Cells
09:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
이 문서는 자세한 microinjection 기술을 사용하여 편광 상피 세포의 작은 GTPases의 overexpression 및 분석에 관련된 절차를.
Tags
Small GTPases
Microinjection
Plasmids
Polarized Epithelial Cells
Apical Domain
Basolateral Domain
Tight Junctions
MDCK Cells
Cell Polarity
Ras Superfamily
RalA
Cdc42
Rab8
Rab10
Rab13
GDP-bound State
GTP-bound State
Nucleotide Binding Regions
Dominant Negative
Dominant Active Alleles
Overexpression
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