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Bioengineering
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荧光光谱法测定膜蛋白质折叠的热力学
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
Please note that all translations are automatically generated.
Click here for the English version.
荧光光谱法测定膜蛋白质折叠的热力学
DOI:
10.3791/2669-v
•
10:09 min
•
April 28, 2011
•
Diana E. Schlamadinger
,
Judy E. Kim
1
Chemistry and Biochemistry
,
University of California San Diego – UCSD
Chapters
00:05
Title
00:44
Introduction
01:24
Preparation of ~50 nm Diameter Small Unilamellar Vesicles (SUVs) for Membrane Protein Folding
03:10
Sample Preparation for Initial Fluorescence Unfolding Curve
04:34
Measurement of Fluorescence Spectra
05:45
Generation of Initial Unfolding Curve and Estimation of Gibbs Free Energy of Membrane Protein Unfolding
08:21
Representative Results for the Measurement of Membrane Protein Unfolding
09:22
Conclusion
Summary
Automatic Translation
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此视频文章详细介绍了实验过程中色氨酸荧光膜蛋白质折叠吉布斯自由能获得。
Tags
Thermodynamics
Membrane Protein Folding
Fluorescence Spectroscopy
Gibbs Free Energy
Unfolding
Denaturant
Integral Membrane Protein
E. Coli
Equilibrium Populations
Folded States
Unfolded States
Denaturant Concentration
Lipid Vesicles
Data Analysis Procedure
Temperature
PH
Folding Environments
Lipids
Micelles
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