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الديناميكا الحرارية من البروتين للطي غشاء المقاسة بواسطة التحليل الطيفي الإسفار
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Chapters
- 00:05Title
- 00:44Introduction
- 01:24Preparation of ~50 nm Diameter Small Unilamellar Vesicles (SUVs) for Membrane Protein Folding
- 03:10Sample Preparation for Initial Fluorescence Unfolding Curve
- 04:34Measurement of Fluorescence Spectra
- 05:45Generation of Initial Unfolding Curve and Estimation of Gibbs Free Energy of Membrane Protein Unfolding
- 08:21Representative Results for the Measurement of Membrane Protein Unfolding
- 09:22Conclusion
