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荧光光谱法测定膜蛋白质折叠的热力学
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
DOI:

10:09 min

April 28, 2011

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Chapters

  • 00:05Title
  • 00:44Introduction
  • 01:24Preparation of ~50 nm Diameter Small Unilamellar Vesicles (SUVs) for Membrane Protein Folding
  • 03:10Sample Preparation for Initial Fluorescence Unfolding Curve
  • 04:34Measurement of Fluorescence Spectra
  • 05:45Generation of Initial Unfolding Curve and Estimation of Gibbs Free Energy of Membrane Protein Unfolding
  • 08:21Representative Results for the Measurement of Membrane Protein Unfolding
  • 09:22Conclusion

Summary

Automatic Translation

此视频文章详细介绍了实验过程中色氨酸荧光膜蛋白质折叠吉布斯自由能获得。

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