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막 단백질 폴딩의 열역학은 형광 분광학에 의해 측정
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
DOI:

10:09 min

April 28, 2011

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Chapters

  • 00:05Title
  • 00:44Introduction
  • 01:24Preparation of ~50 nm Diameter Small Unilamellar Vesicles (SUVs) for Membrane Protein Folding
  • 03:10Sample Preparation for Initial Fluorescence Unfolding Curve
  • 04:34Measurement of Fluorescence Spectra
  • 05:45Generation of Initial Unfolding Curve and Estimation of Gibbs Free Energy of Membrane Protein Unfolding
  • 08:21Representative Results for the Measurement of Membrane Protein Unfolding
  • 09:22Conclusion

Summary

Automatic Translation

트립토판 형광으로 막 단백질 폴딩의 깁스 자유 에너지를 얻기 위해이 동영상 기사 세부 실험 절차.

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