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从废弃的人类胎儿大脑皮质组织神经干细胞的生成
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Neuroscience
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JoVE Journal Neuroscience
Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
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从废弃的人类胎儿大脑皮质组织神经干细胞的生成

DOI:
10.3791/2681-v

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07:29 min

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May 25, 2011

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, , , ,
1Department of Neurology,Beth Israel Deaconess Medical Center, 2Department of Obstetrics and Gynecology,Brigham and Women’s Hospital, 3Department of Pathology,Beth Israel Deaconess Medical Center, 4Department of Pathology, Division of Neuropathology,Brigham and Women’s Hospital

Chapters

  • 00:05Title
  • 01:13Preparation of Solutions and Materials
  • 02:08Isolating Human Neural Stem Cells from the Human Fetal Brain
  • 04:27Differentiating or Storing the Neural Stem Cells
  • 06:23Transfected and Differentiated Neurospheres
  • 06:53Conclusion

Summary

Automatic Translation

Automatic Translation

描述一个简单和可靠的方法是从丢弃的人类胚胎皮层组织的神经干细胞的分离和培养。从已知的人类神经系统疾病的病理细胞和分子过程的特性，可用于文化，以及提供一个平台，以评估药理药效。

Tags

Neural Stem CellsNSCsNeuroepitheliumCortical PlateIntermediate ProgenitorsNeuronal CellsGlial CellsCerebral CortexNormal Human NSC DevelopmentNeurological DisordersProgenitor ProliferationMigrationDifferentiationDown Syndrome NSCsAlzheimer’s Disease PhenotypeGenes Located On Human Chromosome 21DissectionCell SortingPlatingPassaging Of Human NSCsDifferentiation Of Human NSCs

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