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使用CD71/TER119流流式细胞仪检测小鼠红系祖细胞的识别和分析
JoVE Journal
Biology
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JoVE Journal Biology
Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
DOI:

15:32 min

August 05, 2011

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Chapters

  • 00:05Title
  • 02:10Harvesting of Tissues
  • 03:03Preparation of Fetal Liver Cells
  • 05:01Antibody Staining for Flow Cytometry
  • 07:14Identification and Analysis of Erythroid Progenitors
  • 13:52Conclusion

Summary

Automatic Translation

一个流式细胞仪方法,鉴定和分化阶段特异性的小鼠红系祖细胞和前体,直接在新鲜收获的小鼠骨髓,脾脏或胎肝的分子分析流。检测依赖于细胞表面标志物CD71,Ter119,细胞大小。

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