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Chapters
- 00:05Title
- 02:10Harvesting of Tissues
- 03:03Preparation of Fetal Liver Cells
- 05:01Antibody Staining for Flow Cytometry
- 07:14Identification and Analysis of Erythroid Progenitors
- 13:52Conclusion
Ett flöde-cytometrisk metod för identifiering och molekylär analys av differentiering-stegs-specifika murin erytroida progenitorer och prekursorer, direkt i nyskördade mus benmärg, mjälte eller lever hos foster. Analysen bygger på cellytan markörer CD71, Ter119 och cellstorlek.
Tags
IdentificationAnalysisMouse Erythroid ProgenitorsCD71/TER119 Flow-cytometric AssayErythropoiesisRed CellsHematopoietic ProgenitorsErythropoietinErythropoietic Stress ResponseCirculating Red CellsErythropoietic Stress ResponsePhysiological ConditionsPathological ConditionsMouse ModelDefinitive ErythropoiesisFetal LiverNeonatal SpleenAdult SpleenBone Marrow
