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使用CD71/TER119流流式细胞仪检测小鼠红系祖细胞的识别和分析
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JoVE Journal
Biology
Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
Please note that all translations are automatically generated.
Click here for the English version.
使用CD71/TER119流流式细胞仪检测小鼠红系祖细胞的识别和分析
DOI:
10.3791/2809-v
•
15:32 min
•
August 05, 2011
•
Miroslav Koulnis*
1
,
Ramona Pop*
1
,
Ermelinda Porpiglia*
1
,
Jeffrey R. Shearstone*
1
,
Daniel Hidalgo
,
Merav Socolovsky
1
Department of Pediatrics and Department of Cancer Biology
,
University of Massachusetts Medical School
Chapters
00:05
Title
02:10
Harvesting of Tissues
03:03
Preparation of Fetal Liver Cells
05:01
Antibody Staining for Flow Cytometry
07:14
Identification and Analysis of Erythroid Progenitors
13:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个流式细胞仪方法,鉴定和分化阶段特异性的小鼠红系祖细胞和前体,直接在新鲜收获的小鼠骨髓,脾脏或胎肝的分子分析流。检测依赖于细胞表面标志物CD71,Ter119,细胞大小。
Tags
Identification
Analysis
Mouse Erythroid Progenitors
CD71/TER119 Flow-cytometric Assay
Erythropoiesis
Red Cells
Hematopoietic Progenitors
Erythropoietin
Erythropoietic Stress Response
Circulating Red Cells
Erythropoietic Stress Response
Physiological Conditions
Pathological Conditions
Mouse Model
Definitive Erythropoiesis
Fetal Liver
Neonatal Spleen
Adult Spleen
Bone Marrow
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