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Journal
/
Neuroscience
/
在
发展中国家小鼠视网膜
体内
电穿孔
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
In vivo
Electroporation of Developing Mouse Retina
Please note that all translations are automatically generated.
Click here for the English version.
在
发展中国家小鼠视网膜
体内
电穿孔
DOI:
10.3791/2847-v
•
05:53 min
•
June 24, 2011
•
Jimmy de Melo
,
Seth Blackshaw
2,3,4,5
1
Solomon H. Snyder Department of Neuroscience
,
Johns Hopkins School of Medicine
,
2
Department of Neurology
,
Johns Hopkins School of Medicine
,
3
Department of Ophthalmology
,
Johns Hopkins School of Medicine
,
4
Center for High-Throughput Biology
,
Johns Hopkins School of Medicine
,
5
Institute for Cell Engineering
,
Johns Hopkins School of Medicine
Chapters
00:05
Title
01:52
Concentration and Subretinal Injection of Plasmid DNA
03:46
Electroporation
03:46
Neonatal Mouse Retinas Electroporated with pCAG-EGFP Over Developmental Time
05:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
一个执行要么增益或丧失功能的研究目的,方法纳入到小鼠的视网膜细胞的质粒DNA<em>在体内</em>提交。这种方法利用瞬态外部电场的应用诱导细胞质膜的渗透性增加。
Tags
In Vivo Electroporation
Developing Mouse Retina
Gene Targeting
Loss Of Function Knockouts
Gain Of Function Experiment
Cell Fate Specification
Terminal Differentiation
DNA Plasmids
Neonatal Mouse Retina
Electrical Impulses
Plasma Membrane Permeability
Gene Transfer
Hydrophilic Plasma Membrane Pores
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