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Bioengineering
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器官Ⅰ型胶原的含量可延展的平台,以评估细胞行为的一个3维的情况下
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context
Please note that all translations are automatically generated.
Click here for the English version.
器官Ⅰ型胶原的含量可延展的平台,以评估细胞行为的一个3维的情况下
DOI:
10.3791/3089-v
•
10:45 min
•
October 13, 2011
•
Paul Timpson
,
Ewan J. Mcghee
,
Zahra Erami
,
Max Nobis
,
Jean A. Quinn
,
Mike Edward
,
Kurt I. Anderson
1
The Beatson Institute for Cancer Research
,
University of Glasgow
,
2
Section of Dermatology, School of Medicine
,
University of Glasgow
Chapters
00:05
Title
01:44
Establishment of Fibroblast Cultures from Skin Explants
02:57
Stage I: Preparation of Collagen I from Rat Tails
04:55
Stage II: Setting Up the 3D Matrix with Embedded Fibroblasts
06:48
Stage III: Plating Cells of Interest on Top of the Matrix
07:33
Stage IV: Transferring the Matrix to a Grid for Invasion (approximately 0-21 days)
08:38
Stage V: Fixation
09:02
Applications of the Organotypic Assay
10:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述的方法,用于制备一个3 - 维的矩阵组成的I型胶原蛋白和初级人成纤维细胞。器官凝胶作为一个有用的基体,以评估侵入细胞的迁移,因为它模仿组织基质的基本功能,是适合各种形式的显微镜。
Tags
Organotypic Collagen I Assay
Cell Behavior
3-dimensional Context
Cell Migration
Biology
Development
Wound Healing
Immune System
Tumor Cells
Glass Coverslips
Light Microscopy
Local Environment
Elasticity
Protein Composition
Pore Size
Two-dimensional Substrates
Stromal Fibroblasts
Immune Cells
Invasion Of Cancer Cells
Molecular Dynamics
Drug Treatment
response To Drug Treatment
In Vitro And In Vivo Comparison
Cell Derived Matrix
Matrigel
Organotypic Culture
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