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Journal
/
Biology
/
对赫斯特的分离与表征
低
CD45
负
小鼠肺间质干细胞
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolation & Characterization of Hoechst
low
CD45
negative
Mouse Lung Mesenchymal Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
对赫斯特的分离与表征
低
CD45
负
小鼠肺间质干细胞
DOI:
10.3791/3159-v
•
16:55 min
•
October 26, 2011
•
Kelsey S. Chow
2
,
DuHyun Jun
2
,
Karen M. Helm
,
David H. Wagner
2,4
,
Susan M. Majka
2,3
1
Charles C. Gates Regenerative Medicine and Stem Cell Biology Program
,
University of Colorado Denver
,
2
Department of Medicine
,
University of Colorado Denver
,
3
Cancer Center
,
University of Colorado Denver
,
4
Webb Waring Institute
,
University of Colorado Denver
Chapters
00:05
Title
01:49
Lung Isolation
04:05
Preparation of a Single Cell Suspension from Lung Tissue
07:15
Flow Cytometry Analysis to Define and Isolate a Lung MSC Population using Hoechst dye Efflux to Detect a Side Population
13:16
Enumeration of Lung MSC using a Colony Forming Assay (CFU-F)
14:49
Representative Analysis of Isolated MSCs
16:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这篇文章中,我们展示了居民小鼠肺间质干细胞(肺海安),他们的扩张,表征和分析免疫属性的隔离。
Tags
Isolation
Characterization
Hoechstlow CD45negative
Mouse Lung
Mesenchymal Stem Cells
Regulators
Regeneration
Fibrosis
Inflammation
Angiogenesis
Tumor Formation
Injury
Diseases
Pulmonary Fibrosis
Arterial Hypertension
Technology
Define Set Of Markers
Repeated Isolation
Well-characterized Stem Cell Population
Flow Cytometry
Specific Cell Type
Function
Article
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