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原位杂交技术在植物中的精确定位成绩单
JoVE Journal
Biology
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JoVE Journal Biology
In Situ Hybridization for the Precise Localization of Transcripts in Plants
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原位杂交技术在植物中的精确定位成绩单

DOI:
10.3791/3328-v

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12:15 min

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November 23, 2011

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, ,
1Cold Spring Harbor Laboratory

Chapters

  • 00:05Title
  • 01:42Tissue Embedding
  • 02:36Sectioning of Tissue Blocks
  • 04:14In Situ Hybridization
  • 06:30Post-hybridization Treatment
  • 08:27Representative Results of In Situ Hybridization
  • 11:47Conclusion

Summary

Automatic Translation

Automatic Translation

“<em>原位</em>这里描述的杂交协议允许的mRNA和小RNA在细胞水平的高灵敏度和特异性表达的直接本地化。石蜡包埋植物组织切片进行了优化的过程，是适用于广泛的植物和组织，并可以在十天内完成。

Tags

In Situ HybridizationPrecise LocalizationTranscriptsPlantsGenomics ResearchPlant BiologyGene ExpressionMicroarrayNext Generation SequencingDevelopmental ProcessesPhysiological ProcessesStress Response ProcessesEpigenetic PathwaysSmall RNA PathwaysGene Regulatory NetworksSpatiotemporal ResolutionProfiling MethodLaser MicrodissectionFluorescence-activated Cell SortingBiological Role Of A GeneSpatiotemporal Pattern Of ExpressionCellular ResolutionDevelopmentEnvironmental StimuliMutants AnalysisTransgenic Reporter Lines

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