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Chapters
- 00:05Title
- 01:20Automated Adherent Cell Culture Process
- 03:31shRNA Virus Production and Plating onto Assay Plates
- 05:44Lentiviral Transduction and Neuronal Differentiation of SH-SY5Y Cells
- 06:57High Content Image Acquisition and Image Analysis
- 09:27Representative Results of High Throughput Content Screening
- 13:09Conclusion
Noi descriviamo una metodologia che combina coltura automatizzati cella con alto contenuto di imaging per visualizzare e quantificare molteplici processi cellulari e le strutture, in un high-throughput modo. Tali metodi possono aiutare nel ulteriore annotazione funzionale dei genomi, oltre a identificare le reti gene della malattia e potenziali bersagli farmacologici.
Tags
High Content ScreeningNeurodegenerative DiseasesFunctional AnnotationGenomesMolecular NetworksDrug Target IdentificationGenetic Risk FactorsPathogenic MechanismsFunctional ValidationGenome Wide Association StudyParkinson’s DiseaseCausative VariantPathogenic MechanismAssociated RegionGenesPhenotypesTraditional Cell Biology TechniquesMolecular NetworksGenetic MutationsDisease PhenotypesNetwork ComplexityDrug Approval
