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Chapters
- 00:05Title
- 01:20Automated Adherent Cell Culture Process
- 03:31shRNA Virus Production and Plating onto Assay Plates
- 05:44Lentiviral Transduction and Neuronal Differentiation of SH-SY5Y Cells
- 06:57High Content Image Acquisition and Image Analysis
- 09:27Representative Results of High Throughput Content Screening
- 13:09Conclusion
Vi beskriver en metode til at kombinere automatiseret celle dyrkning med høj-indhold billeddannelse til at visualisere og kvantificere flere cellulære processer og strukturer, i en høj-throughput måde. Sådanne metoder kan hjælpe i den videre funktionelle annotation af genomer samt identificere sygdomsgenet netværk og potentielle lægemiddelkandidater.
Tags
High Content ScreeningNeurodegenerative DiseasesFunctional AnnotationGenomesMolecular NetworksDrug Target IdentificationGenetic Risk FactorsPathogenic MechanismsFunctional ValidationGenome Wide Association StudyParkinson’s DiseaseCausative VariantPathogenic MechanismAssociated RegionGenesPhenotypesTraditional Cell Biology TechniquesMolecular NetworksGenetic MutationsDisease PhenotypesNetwork ComplexityDrug Approval
