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Chapters
- 00:05Title
- 01:47PEGDM Synthesis
- 03:12Culture, Infection, and Aggregation of Cells
- 04:42Encapsulation of Cells in PEGDM
- 06:39Hypoxia Tracking
- 07:12Representative Hydrogels and Fluorescent Hypoxic Signaling
- 08:39Conclusion
En metode for foto-innkapsling av celler i en krysskoblet PEG hydrogel er beskrevet. Hypoksisk signalering innenfor innkapslet murine insulinoma (MIN6) aggregater spores ved hjelp av et fluoriserende fargestoff system. Dette systemet tillater seriell undersøkelse av celler i en hydrogel stillas og korrelasjon av hypoksisk signalering med endringer i celle fenotype.
Tags
TrackingHypoxic SignalingEncapsulated Cell AggregatesDiabetes Mellitus Type 1Pancreatic Beta-cellsInsulin ProductionHyperglycemiaExogenous Insulin InjectionTransplantationNormoglycemiaImmune SystemEncapsulationBiologically-derived MaterialsAlginateAgaroseInflammationFibrotic OvergrowthNutrient TransportOxygen TransportSynthetic Poly(ethylene Glycol)PEG-based HydrogelsPhoto-crosslinking ReactionUV DegradationPhotoinitiatorIrgacure 2959Free Radicals
