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Un sistema experimental para estudiar Mecanotransducción en células de pulmón fetal
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Chapters
- 00:05Title
- 00:58Coating Culture Plates with Extra-cellular Matrix Proteins
- 02:15Isolation of Fetal Rodent Lung Type II Epithelial Cells
- 05:32Isolation of Fetal Rodent Lung Fibroblasts
- 06:15Experimental System to Provided Mechanical Stimulation to Lung Cells
- 07:42Representative Results from Mechanotransduction Experiments Using Fetal Lung Cells
- 09:15Conclusion
Las fuerzas mecánicas juegan un papel clave en el desarrollo pulmonar y lesión pulmonar. A continuación, describimos un método para aislar roedores pulmonares fetales células tipo II epiteliales y fibroblastos y exponerlos a la estimulación mecánica mediante un<em> In vitro</em> Sistema.
Tags
Experimental SystemMechanotransductionFetal Lung CellsMechanical ForcesLung DevelopmentAlveolar Type II Epithelial CellsPulmonary SurfactantFluid HomeostasisDistal Lung Parenchyma CellsExaggerated StretchMechanical VentilationMolecular MechanismsCellular MechanismsMechanical StimuliLung InjuryIsolation MethodFlexcell Strain UnitIn Vitro SystemMechanical Stimulation
