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海藻酸钠微胶囊作为一个人类胚胎干细胞(胚胎干细胞)的繁殖和分化的3D平台,以不同的谱系
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JoVE Journal
Bioengineering
Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages
Please note that all translations are automatically generated.
Click here for the English version.
海藻酸钠微胶囊作为一个人类胚胎干细胞(胚胎干细胞)的繁殖和分化的3D平台,以不同的谱系
DOI:
10.3791/3608-v
•
10:01 min
•
March 09, 2012
•
Kuldip Sidhu
,
Jaemin Kim
,
Methichit Chayosumrit
,
Sophia Dean
,
Perminder Sachdev
1
Stem Cell Lab, School of Psychiatry, Faculty of Medicine
,
The University of New South Wales
,
2
Siriraj Center of Excellence for Stem cell Research, Faculty of Medicine Siriraj Hospital
,
Mahidol University
,
3
Neuropsychiatric Institute
,
Prince of Wales Hospital
Chapters
00:05
Title
02:11
Preparation of 1.1% Alginate
02:54
Preparation of hESC for Encapsulation
04:03
Encapsulation of hESC
05:42
Differentiation of Encapsulated hESC to DA Neurons
06:40
Decapsulation of Encapsulated hESC
07:28
Differentiating DA Neurons in a 3D environment
09:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们已经优化了微胶囊技术作为一种有效的繁殖和胚胎干细胞分化为内胚层和多巴胺(DA)神经元的3D平台1。它还提供了一个机会,从宿主细胞移植过程中的免疫隔离。这个平台可以适应其他类型的细胞。
Tags
Alginate Microcapsule
3D Platform
Propagation
Differentiation
Human Embryonic Stem Cells
HESC
Biomaterials
Stem Cell Cultures
Microenvironment
Cell-to-cell Interaction
Cell Proliferation
Specific Lineages
Encapsulation
Semi-permeable Membranes
3D Cultures
Nutrients Exchange
Oxygen Exchange
Stimuli Exchange
Antibodies Exclusion
Immune Cells Exclusion
Alginate Microcapsules
Culture Conditions Enhancement
P160-Rho-associated Coiled-coil Kinase Inhibitor
ROCK Inhibitor
Y-27632
Human Fetal Fibroblast-conditioned Serum Replacement Medium
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