Journal
/
Immunology and Infection
/
High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.

High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.

DOI:
10.3791/3629-v

•

11:46 min

•

March 12, 2012

•

, ,
1Department of Nematology,University of California, Riverside

Chapters

  • 00:05Title
  • 01:43Tomato Seedling Growth in Pots and Trays
  • 02:48Cowpea Growth in Seedling Pouches
  • 03:46Extraction of Nematode Eggs
  • 05:45Hatching Nematode Eggs
  • 06:46Tomato Root Infection in Pots or Trays and Evaluation of Infection
  • 08:42Cowpea Root Infection in Pouches and Evaluation of Infection
  • 10:05Representative Images of Nematode-infected Tomato and Cowpea Roots
  • 10:49Conclusion

Summary

Automatic Translation

Automatic Translation

Two distinct methods to screen plants with root-knot nematodes are described. The described approaches include high-throughput screens with nematodes in a nondestructive manner facilitating the use of these plants in breeding programs.

Tags

Root-knot NematodesMeloidogyne Spp.High-throughput ScreensPlant ParasitesObligate ParasitesEconomically Important NematodesSecond-stage Juvenile (J2)Infective StageSoil MovementRoot PenetrationFeeding Site EstablishmentGiant CellsGall FormationRoot Knot SymptomMoltsAdult StageEgg LayingLife Cycle CompletionTraditional ProtocolPlant InfectionHigh-throughput Assays

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3629?article_type=v

High-throughput Detection Method for Influenza Virus
check_url/3629?article_type=v

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
check_url/3629?article_type=v

ScanLag: High-throughput Quantification of Colony Growth and Lag Time
check_url/3629?article_type=v

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
check_url/3629?article_type=v

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery
check_url/3629?article_type=v

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
check_url/3629?article_type=v

High-throughput Gene Tagging in Trypanosoma brucei
check_url/3629?article_type=v

A High-throughput, High-content, Liquid-based C. elegans Pathosystem
check_url/3629?article_type=v

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
check_url/3629?article_type=v

A High-throughput Shigella-specific Bactericidal Assay

Read Article