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Genoverføring til Developing Mouse indre øret ved<em> In Vivo</em> Electroporation
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Chapters
- 00:05Title
- 01:58Part 1: Ventral Laparotomy
- 05:23Ventral Midline Incision
- 07:46Part 2: Transuterine Microinjection
- 08:32Common Embryo Orientations Observed by Transillumination of the Uterus
- 10:19Embryo Reorientation for Otocyst Injections
- 13:48Transuterine Microinjection
- 16:31Part 3: In vivo Electroporation
- 17:37Postnatal Selection of a Pup whose Otocyst was Injected at E12.5
- 19:47Prophylactic Analgesia
- 20:18Surgical Subject the Morning after Surgery
- 20:55Part 4: Representative Results
Musen indre øret er en placode-avledet sanseorgan som utviklingsmessig programmet er utdypet under svangerskapet. Vi definerer en<em> I fosterlivet</em> Genoverføring teknikk består av tre trinn: mus ventralt laparotomi, transuterine mikroinjeksjon, og<em> In vivo</em> Electroporation. Vi bruker digital video mikroskop for å demonstrere de kritiske eksperimentelle embryologiske teknikker.
Tags
Gene TransferDeveloping Mouse Inner EarIn Vivo ElectroporationSensory EpitheliaCristaeMaculaeOrgan Of CortiVestibular Hair CellsAuditory Hair CellsCell Fate SpecificationMorphogenesisGestationTransgenic EmbryosMolecular BasisGain-of-function StudiesLoss-of-function StudiesGene Misexpression StudiesVentral LaparotomyTransuterine Microinjection
