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工程双层凝胶控制升序分化
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Bioengineering
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JoVE Journal Bioengineering
Engineering a Bilayered Hydrogel to Control ASC Differentiation
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工程双层凝胶控制升序分化

DOI:
10.3791/3953-v

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07:48 min

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May 25, 2012

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, , ,
1Department of Extremity Trauma Research and Regenerative Medicine,United States Army Institute of Surgical Research, 2Department of Biomedical Engineering,The University of Texas at Austin

Chapters

  • 00:05Title
  • 01:54Preparation and Characterization of Adipose-derived Stem Cells-Chitosan Microspheres Embedded in PRG-fibrin Gels
  • 03:40Preparation and Characterization of ASC-CSM Embedded in Collagen Gels
  • 04:34Development of Bilayered PEG-fibrin-(ASC-CSM)-Collagen Gel Constructs
  • 05:54Representative Differentiation Results
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

该协议着重于利用干细胞的固有能力，其周围的细胞外基质的线索，被诱导分化成多个表型。这种方法手稿延伸我们描述一个利用双层水凝胶的模型，PEG-纤维蛋白和胶原蛋白的组成和特性，同时脂肪干细胞分化<sup> 1</sup>。

Tags

EngineeringBilayered HydrogelASC DifferentiationNatural PolymersHost BiocompatibilityCell InteractionRegenerative MedicineBiomaterialsStem CellsThree-dimensional ScaffoldDecellularized Extracellular MatrixHydrogelsMicro/nano ParticlesCell FunctionSubstrateAdherenceProliferationDifferentiated Cellular PhenotypeExtracellular Matrix (ECM)Reciprocal ConnectionOrganizationAdipose-derived Stem Cells (ASCs)Mesenchymal Stem CellsMulti-lineage DifferentiationCo-culturingBilayered Matrices

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