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Chapters
- 00:05Title
- 01:54Preparation and Characterization of Adipose-derived Stem Cells-Chitosan Microspheres Embedded in PRG-fibrin Gels
- 03:40Preparation and Characterization of ASC-CSM Embedded in Collagen Gels
- 04:34Development of Bilayered PEG-fibrin-(ASC-CSM)-Collagen Gel Constructs
- 05:54Representative Differentiation Results
- 07:02Conclusion
This protocol focuses on utilizing the inherent ability of stem cells to take cue from their surrounding extracellular matrix and be induced to differentiate into multiple phenotypes. This methods manuscript extends our description and characterization of a model utilizing a bilayered hydrogel, composed of PEG-fibrin and collagen, to simultaneously co-differentiate adipose-derived stem cells1.
Tags
EngineeringBilayered HydrogelASC DifferentiationNatural PolymersHost BiocompatibilityCell InteractionRegenerative MedicineBiomaterialsStem CellsThree-dimensional ScaffoldDecellularized Extracellular MatrixHydrogelsMicro/nano ParticlesCell FunctionSubstrateAdherenceProliferationDifferentiated Cellular PhenotypeExtracellular Matrix (ECM)Reciprocal ConnectionOrganizationAdipose-derived Stem Cells (ASCs)Mesenchymal Stem CellsMulti-lineage DifferentiationCo-culturingBilayered Matrices
