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Chapters
- 00:05Title
- 01:54Preparation and Characterization of Adipose-derived Stem Cells-Chitosan Microspheres Embedded in PRG-fibrin Gels
- 03:40Preparation and Characterization of ASC-CSM Embedded in Collagen Gels
- 04:34Development of Bilayered PEG-fibrin-(ASC-CSM)-Collagen Gel Constructs
- 05:54Representative Differentiation Results
- 07:02Conclusion
Ce protocole met l'accent sur l'utilisation de la capacité inhérente de cellules souches de prendre exemple sur leur matrice extracellulaire environnante et être amené à se différencier en de multiples phénotypes. Ce manuscrit méthodes étend notre description et la caractérisation d'un modèle utilisant un hydrogel bicouche, composé de PEG-fibrine et le collagène, à la fois co-différencier les cellules souches adipeuses<sup> 1</sup>.
Tags
EngineeringBilayered HydrogelASC DifferentiationNatural PolymersHost BiocompatibilityCell InteractionRegenerative MedicineBiomaterialsStem CellsThree-dimensional ScaffoldDecellularized Extracellular MatrixHydrogelsMicro/nano ParticlesCell FunctionSubstrateAdherenceProliferationDifferentiated Cellular PhenotypeExtracellular Matrix (ECM)Reciprocal ConnectionOrganizationAdipose-derived Stem Cells (ASCs)Mesenchymal Stem CellsMulti-lineage DifferentiationCo-culturingBilayered Matrices
