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Chapters
- 00:05Title
- 01:22Oocyte Collection
- 03:09Agarose Embedding and Lysis
- 04:41Bisulfite Mutagenesis
- 07:00First and Second Round PCR Amplification
- 09:09TA Cloning and Colony PCR
- 10:31Results: Electrophoretic Analysis of Single Oocyte Bisulfite Mutagenesis
- 12:40Conclusion
Tags
Single Oocyte Bisulfite MutagenesisEpigeneticsChromatin ModificationsGene AccessibilityGene TranscriptionDNA MethylationRepressive MarkCpG DinucleotidesMethyl GroupRepressive ProteinsHistone ModificationsDevelopmental ProgrammingCell DifferentiationRetroviral ElementsX-chromosome InactivationGenomic ImprintingBisulfite MutagenesisSodium BisulfiteDeaminationUracilsPCR AmplificationSequencingConversion EventsThyminesMethylated CytosinesNucleotide LevelAgarose Bead
