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Journal
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Biology
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单卵母细胞的亚硫酸氢钠诱变
JoVE Journal
Biology
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JoVE Journal
Biology
Single Oocyte Bisulfite Mutagenesis
Please note that all translations are automatically generated.
Click here for the English version.
单卵母细胞的亚硫酸氢钠诱变
DOI:
10.3791/4046-v
•
13:18 min
•
June 27, 2012
•
Michelle M. Denomme
2,3
,
Liyue Zhang
,
Mellissa R.W. Mann
2,3
1
Department of Obstretrics & Gynaecology
,
Schulich School of Medicine and Dentistry, University of Western Ontario
,
2
Department of Biochemistry
,
Schulich School of Medicine and Dentistry, University of Western Ontario
,
3
Children’s Health Research Institute
Chapters
00:05
Title
01:22
Oocyte Collection
03:09
Agarose Embedding and Lysis
04:41
Bisulfite Mutagenesis
07:00
First and Second Round PCR Amplification
09:09
TA Cloning and Colony PCR
10:31
Results: Electrophoretic Analysis of Single Oocyte Bisulfite Mutagenesis
12:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
亚硫酸氢钠诱变是分析DNA甲基化的黄金标准。我们修改后的协议允许在单细胞水平和DNA甲基化分析,是专门设计为个别的卵母细胞。它也可以用于卵裂阶段的胚胎。
Tags
Single Oocyte Bisulfite Mutagenesis
Epigenetics
Chromatin Modifications
Gene Accessibility
Gene Transcription
DNA Methylation
Repressive Mark
CpG Dinucleotides
Methyl Group
Repressive Proteins
Histone Modifications
Developmental Programming
Cell Differentiation
Retroviral Elements
X-chromosome Inactivation
Genomic Imprinting
Bisulfite Mutagenesis
Sodium Bisulfite
Deamination
Uracils
PCR Amplification
Sequencing
Conversion Events
Thymines
Methylated Cytosines
Nucleotide Level
Agarose Bead
Article
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