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基于DNA载体的RNA干扰在癌症研究基因功能
JoVE Journal
Medicine
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JoVE Journal
Medicine
DNA Vector-based RNA Interference to Study Gene Function in Cancer
Please note that all translations are automatically generated.
Click here for the English version.
基于DNA载体的RNA干扰在癌症研究基因功能
DOI:
10.3791/4129-v
•
13:10 min
•
June 04, 2012
•
Daniel B. Stovall
,
Meimei Wan
,
Qiang Zhang
,
Purnima Dubey
,
Guangchao Sui
1
Department of Cancer Biology and Comprehensive Cancer Center
,
Wake Forest University School of Medicine
,
2
Department of Pathology and Comprehensive Cancer Center
,
Wake Forest University School of Medicine
Chapters
00:05
Title
01:54
Generation of shRNA Constructs
04:33
Lentivirus Transfection
06:35
Infection and Characterization of Infected Cells
07:47
Xenograft Mouse Model Study
10:39
YY1 Knockdown Experiment
12:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
RNA干扰(RNAi)拥有超过基因敲除的许多优点,在基因功能研究的工具已被广泛使用。基于DNA载体的RNAi技术的发明,已作出长期和诱导基因敲除可能,也增加了基因沉默的可行性<em>在体内</em>。
Tags
DNA Vector-based
RNA Interference
Gene Function
Cancer Research
SiRNA
Gene Silencing
RNAi Technology
Small Hairpin RNA (shRNA)
U6 Promoter
H1 Promoter
Lentivirus
Lentiviral Vectors
Cell Infection
Mouse Xenograft Model
ShRNA Constructs
PCR Amplification
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