A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Medications Development, Ernest Gallo Clinic and Research Center,University of California, San Francisco , 2Clinical Pharmacology and Experimental Therapeutics,University of California, San Francisco , 3Translational Research Institute and the Institute for Health and Biomedical Innovation,Queensland University of Technology, Brisbane, Australia
Chapters
- 00:05Title
- 01:19Cell Treatment
- 04:36Acquisition of Three-dimensional Fluorescence Images
- 05:26Three-dimensional Morphometric Analysis
- 07:31Results: Single-cell Phenotypic Changes Visualized Through Three-dimensional Morphometric Analysis
- 09:29Conclusion
Vi utviklet en programvare-plattform som utnytter Imaris Neuroscience, ImarisXT og MATLAB for å måle endringer i morfologi av en udefinert form hentet fra tre-dimensjonale confocal fluorescens av enkeltceller. Denne romanen tilnærmingen kan brukes til å kvantifisere endringer i cellen form etter reseptor aktivering og representerer derfor en mulig ekstra verktøy for medisiner.
Tags
Analytical ToolCellular Morphology ChangesThree-dimensional Fluorescence ImagesSoftware Analysis ToolsFluorescence Image Measurement3D DatasetsCellular DynamicsCellular PlasticityMicroscopy InstrumentsMultispectral Fluorescence ImagesConfocal Stacks3D RepresentationDesign-based Stereology MethodsAutomated Analytic MethodCell Morphology AnalysisProtein Localization AnalysisReceptor Trafficking AnalysisMeta-Morph
