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1Research Service,Veterans Affairs Greater Los Angeles Healthcare System, 2Department of Urology, The David Geffen School of Medicine,University of California, Los Angeles , 3GeneFluidics, 4Division of Infectious Diseases,Veterans Affairs Greater Los Angeles Healthcare System, 5Department of Microbiology, Immunology & Molecular Genetics,University of California, Los Angeles
Chapters
- 00:05Title
- 01:39Functionalization of Electrochemical Sensors
- 03:02Sample Preparation
- 03:55Electrochemical Sensor Assay
- 05:14Results: Bacterial Detection & Identification Using Electrochemical Sensors
- 08:46Conclusion
Vi beskriver en elektrokemisk metod sensor analys för snabb bakteriell detektion och identifiering. Analysen omfattar en sensor array med funktionella fånga DNA oligonukleotidsonder för ribosom RNA (rRNA) artspecifika sekvenser. Sandwich hybridisering av mål-rRNA med infångningsproben och en pepparrotsperoxidas-länkad DNA oligonukleotid detektorsonden ger en mätbar amperometriskt ström.
Tags
Bacterial DetectionIdentificationElectrochemical SensorsBlood Glucose MeasurementAnalyte DetectionSignal TransductionRedox EnzymeAmperometric ElectrodeSensor SpecificityGlucose SensorDNA OligonucleotidesSandwich HybridizationRibosomal RNA (rRNA)Capture ProbeDetector ProbeHorseradish Peroxidase (HRP)Substrate OxidationCurrent Flow
