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En enkel protokoll for blodplater-mediert klumping av<em> Plasmodium falciparum</em>-Infiserte Erytrocytter i en ressurs Dårlig innstilling
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

En enkel protokoll for blodplater-mediert klumping av<em> Plasmodium falciparum</em>-Infiserte Erytrocytter i en ressurs Dårlig innstilling

DOI:
10.3791/4316-v

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07:27 min

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May 16, 2013

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, , ,
1Malawi-Liverpool-Wellcome Trust Clinical Research Programme, 2Liverpool School of Tropical Medicine, 3Department of Microbiology, Division of Medical Parasitology,New York University School of Medicine

Chapters

  • 00:05Title
  • 01:20Preparaing of Platelet-rich Plasma (PRP) and Platelet-poor Plasma (PPP)
  • 02:17Parasite Culture
  • 02:58Thin Blood Film Slide Preparation
  • 03:57Plasmagel Flotation
  • 05:01Clumping Assay
  • 06:08Results: Platelet-mediated Clump Assay
  • 06:57Conclusion

Summary

Automatic Translation

Automatic Translation

Denne metoden undersøker blodplater-mediert klumper fenotype av<em> Plasmodium falciparum</em>-Infiserte erytrocytter (PRBC) i kliniske isolater. Dette blir utført ved å isolere og co-inkubering av blodplate-rikt plasma, og en suspensjon av PRBC.

Tags

Platelet-mediated ClumpingPlasmodium Falciparum-infected ErythrocytesResource Poor SettingCerebral MalariaMicrovessels ObstructionP. Falciparum-parasitized Red Blood CellsSequestrationEndothelial CellsPost-capillary VenulesSplenic ClearanceLow Oxygen PressureCytoadherenceHost ReceptorsSevere Pathological Outcomes

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