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Chapters
- 00:05Title
- 01:19Obtaining and Preparing the Tissue
- 02:22Mounting the Tissue
- 03:38Stimulation
- 04:31Harvesting
- 05:11Mucosal Explants Responses to Pro-Inflammatory Stimulus
- 05:58Conclusion
Vi introduserer en ny fremgangsmåte for vedlikehold av human intestinal mukosa i kultur og overvåking av responsen på ulike typer stimuli over minst 24 timer. Med foreliggende fremgangsmåte, er polariteten av vev opprettholdes, slik at for en fysiologisk stimulering via den apikale rute.
Tags
Human Intestinal Mucosa ExplantsIntestinal Micro-environmentImmunological ResponseTolerance Against Food AntigensImmune Responses Against Pathogenic MicrobesMicrobiota-host InteractionsMucus DegradationAntimicrobial PeptidesEpithelial BarrierGut-resident Lymphoid CellsIn Vitro AssaysMouse ModelsIrritable Bowel SyndromeInflammatory Bowel DiseaseColorectal Cancer
